Olson A D
Department of Pediatrics, Schneider Children's Hospital, Stony Brook, New York.
Dig Dis Sci. 1993 Mar;38(3):388-95. doi: 10.1007/BF01316489.
We have developed a model system to quantify the tractional forces generated by intestinal epithelial cells during organization into a confluent epithelial cell sheet. In this model system, IEC-6 cells, a rat intestinal crypt cell line, rapidly contracted collagen gels reducing the gel surface area by 97% at 24 hr. The tractional forces measured by gel contraction were directly related to the number of cells added and were inversely related to the collagen concentration of the gel. Actin microfilament function was required for gel contraction, but microtubular function was not. Fetal bovine serum and protein synthesis were required for maximal gel contraction. IEC-6 (5 x 10(5)) cells per gel and fibroblasts (5 x 10(4)) cells added to collagen gels resulted in contraction of the gels by 50% at 24 hr. Therefore, intestinal epithelial cells and fibroblasts generate tractional forces of similar strength capable of organizing the surrounding extracellular matrix, which should be considered in models of intestinal morphogenesis and repair.
我们开发了一个模型系统，用于量化肠上皮细胞在组织形成汇合上皮细胞片过程中产生的牵引力。在这个模型系统中，IEC-6细胞（一种大鼠肠隐窝细胞系）能迅速收缩胶原凝胶，在24小时时使凝胶表面积减少97%。通过凝胶收缩测量的牵引力与添加的细胞数量直接相关，与凝胶的胶原浓度呈负相关。凝胶收缩需要肌动蛋白微丝功能，但不需要微管功能。最大程度的凝胶收缩需要胎牛血清和蛋白质合成。每凝胶加入5×10⁵个IEC-6细胞和5×10⁴个成纤维细胞，在24小时时会使凝胶收缩50%。因此，肠上皮细胞和成纤维细胞产生的牵引力强度相似，能够组织周围的细胞外基质，这在肠形态发生和修复模型中应予以考虑。
