Kruk P A, Auersperg N
Department of Anatomy, University of British Columbia, Vancouver, Canada.
Am J Obstet Gynecol. 1992 Nov;167(5):1437-43. doi: 10.1016/s0002-9378(11)91730-x.
After ovulation the human ovarian surface epithelium proliferates at the wound edges, migrates over the ovulatory defect, and contributes to its repair primarily by the action of proteolytic enzymes and by the deposition of new matrix material. We examined the potential for human ovarian surface epithelial cells to physically remodel extracellular matrix in culture, similar to collagen gel lattice contraction by fibroblasts, a well-known culture model for wound repair, as an additional role of human ovarian surface epithelium in wound repair.
Human ovarian surface epithelium cells from ovarian biopsies of 11 patients were grown in culture and plated onto a combination of collagen gel and rat ovarian surface epithelial-derived extracellular matrix. The degree of matrix contraction was measured as the percentage of the original culture diameter.
Human ovarian surface epithelial cells surrounded and contracted the combination of matrices into a dense matrix organoid. The degree of organoid contraction was related to the number of human ovarian surface epithelial cells plated per organoid and to the inclusion of fibroblasts within the collagen gel but was not affected either by adding epidermal growth factor and hydrocortisone to the culture medium or by reducing the serum component of the medium.
Human ovarian surface epithelial organoids may be useful for the study of normal and abnormal ovarian events such as ovulatory wound repair and cyst formation.
排卵后，人卵巢表面上皮在伤口边缘增殖，迁移至排卵缺损处，并主要通过蛋白水解酶的作用和新基质材料的沉积来促进其修复。我们研究了人卵巢表面上皮细胞在培养中对细胞外基质进行物理重塑的潜力，类似于成纤维细胞引起的胶原凝胶格子收缩，这是一种众所周知的伤口修复培养模型，作为人卵巢表面上皮在伤口修复中的额外作用。
从11名患者的卵巢活检中获取人卵巢表面上皮细胞，进行培养并接种到胶原凝胶和大鼠卵巢表面上皮衍生的细胞外基质的组合上。基质收缩程度以原始培养直径的百分比来衡量。
人卵巢表面上皮细胞包围并将基质组合收缩成致密的基质类器官。类器官收缩程度与每个类器官接种的人卵巢表面上皮细胞数量以及胶原凝胶中是否包含成纤维细胞有关，但不受向培养基中添加表皮生长因子和氢化可的松或减少培养基血清成分的影响。
人卵巢表面上皮类器官可能有助于研究正常和异常的卵巢事件，如排卵伤口修复和囊肿形成。
