Selle H, Chapman B E, Kuchel P W
Department of Biochemistry, University of Sydney, Australia.
Eur J Biochem. 1993 Mar 1;212(2):411-6. doi: 10.1111/j.1432-1033.1993.tb17676.x.
The direct techniques of 1H spin-echo and 31P-NMR spectroscopy made it possible to monitor the release of glycerophosphocholine from lysophosphatidylcholine in lysates from human red blood cells. Thus, the existence of a lysophospholipase in human erythrocytes was confirmed using a new more direct method. No evidence for a phospholipase A2 activity in the haemolysates was found with the same approach; since this enzyme is present in leukocytes, the absence of activity helped verify the purity of the erythrocyte preparation. The lysophospholipase may constitute, with the earlier described glycerophosphocholine phosphodiesterase activity, a metabolic unit for the removal of haemolytic lysophosphatidylcholine which is formed in the erythrocyte membranes as well as taken up from the plasma.
1H 自旋回波和 31P - NMR 光谱的直接技术使得监测甘油磷酸胆碱从人红细胞裂解物中的溶血磷脂酰胆碱释放成为可能。因此，使用一种新的更直接的方法证实了人红细胞中存在溶血磷脂酶。用同样的方法未发现溶血产物中有磷脂酶 A2 活性；由于这种酶存在于白细胞中，活性的缺失有助于验证红细胞制剂的纯度。溶血磷脂酶可能与先前描述的甘油磷酸胆碱磷酸二酯酶活性一起，构成一个代谢单元，用于清除在红细胞膜中形成以及从血浆中摄取的溶血磷脂酰胆碱。
