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大肠杆菌的SecB、SecA和SecY蛋白是细菌素释放蛋白（一种小脂蛋白）表达和膜插入所必需的。

Escherichia coli SecB, SecA, and SecY proteins are required for expression and membrane insertion of the bacteriocin release protein, a small lipoprotein.

作者信息

Oudega B, Mol O, van Ulsen P, Stegehuis F, van der Wal F J, Luirink J

机构信息

Department of Molecular Microbiology, Faculty of Biology, Vrije Universiteit, Amsterdam, The Netherlands.

出版信息

J Bacteriol. 1993 Mar;175(5):1543-7. doi: 10.1128/jb.175.5.1543-1547.1993.

DOI:10.1128/jb.175.5.1543-1547.1993
PMID:8444816
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC193245/
Abstract

The SecB, SecA, and SecY dependency of a small outer membrane lipoprotein in Escherichia coli, the bacteriocin release protein (BRP), was studied. The detrimental effect of BRP expression on the culture turbidity (quasi-lysis) was strongly reduced in the sec mutants. Immunoblotting and radioactive labeling experiments showed that the expression, membrane insertion, and processing of the BRP precursor are dependent on SecB, SecA, and SecY. Labeling experiments with hybrid BRP gene constructs revealed that the mature part of the BRP precursor and not its stable signal sequence is important for its SecB dependency.

摘要

对大肠杆菌中的一种小外膜脂蛋白——细菌素释放蛋白（BRP）的SecB、SecA和SecY依赖性进行了研究。在sec突变体中，BRP表达对培养物浊度（准裂解）的有害影响显著降低。免疫印迹和放射性标记实验表明，BRP前体的表达、膜插入和加工依赖于SecB、SecA和SecY。用杂交BRP基因构建体进行的标记实验表明，BRP前体的成熟部分而非其稳定的信号序列对其SecB依赖性很重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99cb/193245/2f1fa631b4d5/jbacter00047-0346-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99cb/193245/a461bb9fd8e4/jbacter00047-0345-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99cb/193245/fc1232bacf7e/jbacter00047-0345-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99cb/193245/2f1fa631b4d5/jbacter00047-0346-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99cb/193245/a461bb9fd8e4/jbacter00047-0345-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99cb/193245/fc1232bacf7e/jbacter00047-0345-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99cb/193245/2f1fa631b4d5/jbacter00047-0346-a.jpg

相似文献

1
Escherichia coli SecB, SecA, and SecY proteins are required for expression and membrane insertion of the bacteriocin release protein, a small lipoprotein.大肠杆菌的SecB、SecA和SecY蛋白是细菌素释放蛋白（一种小脂蛋白）表达和膜插入所必需的。
J Bacteriol. 1993 Mar;175(5):1543-7. doi: 10.1128/jb.175.5.1543-1547.1993.
2
Preprotein transfer to the Escherichia coli translocase requires the co-operative binding of SecB and the signal sequence to SecA.前体蛋白转移至大肠杆菌转位酶需要SecB和信号序列与SecA协同结合。
Mol Microbiol. 1998 Sep;29(5):1179-90. doi: 10.1046/j.1365-2958.1998.00997.x.
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Overproduction of SecA suppresses the export defect caused by a mutation in the gene encoding the Escherichia coli export chaperone secB.SecA的过量产生抑制了由编码大肠杆菌输出伴侣蛋白secB的基因突变所导致的输出缺陷。
J Bacteriol. 1999 May;181(10):3010-7. doi: 10.1128/JB.181.10.3010-3017.1999.
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SecY and SecA interact to allow SecA insertion and protein translocation across the Escherichia coli plasma membrane.SecY和SecA相互作用，使SecA插入并使蛋白质穿过大肠杆菌质膜进行转运。
EMBO J. 1997 Nov 3;16(21):6384-93. doi: 10.1093/emboj/16.21.6384.
5
[Interaction of SecB and SecA with the N-terminal region of mature alkaline phosphatase on its secretion in Escherichia coli].[SecB和SecA与成熟碱性磷酸酶N端区域在大肠杆菌中分泌过程中的相互作用]
Mol Biol (Mosk). 2003 Jul-Aug;37(4):712-8.
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Membrane insertion of the mannitol permease of Escherichia coli occurs under conditions of impaired SecA function.大肠杆菌甘露醇通透酶的膜插入在SecA功能受损的条件下发生。
J Biol Chem. 1992 Dec 5;267(34):24523-32.
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Identification of a region of interaction between Escherichia coli SecA and SecY proteins.大肠杆菌SecA蛋白与SecY蛋白之间相互作用区域的鉴定。
J Biol Chem. 1997 Apr 25;272(17):11302-6. doi: 10.1074/jbc.272.17.11302.
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A mutation of Escherichia coli SecA protein that partially compensates for the absence of SecB.一种大肠杆菌SecA蛋白的突变，可部分补偿SecB缺失的影响。
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Precursor-specific requirements for SecA, SecB, and delta muH+ during protein export of Escherichia coli.大肠杆菌蛋白质输出过程中SecA、SecB和ΔμH⁺对前体的特异性要求。
J Biol Chem. 1994 Apr 29;269(17):12840-5.
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The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membrane.SecB与SecA再到SecY/E的结合级联反应介导前体蛋白靶向大肠杆菌质膜。
Cell. 1990 Oct 19;63(2):269-79. doi: 10.1016/0092-8674(90)90160-g.

引用本文的文献

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The Agrobacterium tumefaciens virB7 gene product, a proposed component of the T-complex transport apparatus, is a membrane-associated lipoprotein exposed at the periplasmic surface.根癌土壤杆菌virB7基因产物是T复合体转运装置的一个假定组成部分，是一种暴露于周质表面的膜相关脂蛋白。
J Bacteriol. 1996 Jun;178(11):3156-67. doi: 10.1128/jb.178.11.3156-3167.1996.
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Effects of temperature and of heat shock on the expression and action of the colicin A lysis protein.

本文引用的文献

1
E. coli mutant pleiotropically defective in the export of secreted proteins.大肠杆菌突变体在分泌蛋白输出方面存在多效性缺陷。
Cell. 1981 Sep;25(3):765-72. doi: 10.1016/0092-8674(81)90184-7.
2
Regulation of a membrane component required for protein secretion in Escherichia coli.大肠杆菌中蛋白质分泌所需膜成分的调控。
Cell. 1982 Aug;30(1):311-9. doi: 10.1016/0092-8674(82)90037-x.
3
A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.
温度及热休克对大肠杆菌素A裂解蛋白表达及作用的影响
J Bacteriol. 1995 Sep;177(17):5189-92. doi: 10.1128/jb.177.17.5189-5192.1995.
大肠杆菌spc核糖体蛋白操纵子中蛋白质输出基因的特定突变：一种新的温度敏感型secY突变体的分离与鉴定
EMBO J. 1984 Mar;3(3):631-5. doi: 10.1002/j.1460-2075.1984.tb01859.x.
4
Evidence for specificity at an early step in protein export in Escherichia coli.大肠杆菌中蛋白质输出早期步骤特异性的证据。
J Bacteriol. 1985 Jul;163(1):267-74. doi: 10.1128/jb.163.1.267-274.1985.
5
Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli.两个sec基因对蛋白质组装进入大肠杆菌质膜的影响。
J Biol Chem. 1985 Feb 10;260(3):1836-41.
6
Lipoprotein nature of the colicin A lysis protein: effect of amino acid substitutions at the site of modification and processing.大肠杆菌素A裂解蛋白的脂蛋白性质：修饰和加工位点氨基酸取代的影响
J Bacteriol. 1987 May;169(5):2187-94. doi: 10.1128/jb.169.5.2187-2194.1987.
7
Uncoupling of synthesis and release of cloacin DF13 and its immunity protein by Escherichia coli.大肠杆菌对绿脓菌素DF13及其免疫蛋白合成与释放的解偶联作用。
Mol Gen Genet. 1987 Jan;206(1):126-32. doi: 10.1007/BF00326547.
8
Modification, processing, and subcellular localization in Escherichia coli of the pCloDF13-encoded bacteriocin release protein fused to the mature portion of beta-lactamase.与β-内酰胺酶成熟部分融合的pCloDF13编码的细菌素释放蛋白在大肠杆菌中的修饰、加工及亚细胞定位
J Bacteriol. 1987 May;169(5):2245-50. doi: 10.1128/jb.169.5.2245-2250.1987.
9
Production and release of cloacin DF13 and related colicins.克洛acin DF13及相关大肠杆菌素的产生与释放。
Curr Top Microbiol Immunol. 1986;125:183-205. doi: 10.1007/978-3-642-71251-7_11.
10
An unmodified form of the ColE2 lysis protein, an envelope lipoprotein, retains reduced ability to promote colicin E2 release and lysis of producing cells.大肠杆菌素E2裂解蛋白的未修饰形式是一种包膜脂蛋白，其促进大肠杆菌素E2释放和产生菌细胞裂解的能力有所降低。
J Gen Microbiol. 1987 Sep;133(9):2411-20. doi: 10.1099/00221287-133-9-2411.