• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

与β-内酰胺酶成熟部分融合的pCloDF13编码的细菌素释放蛋白在大肠杆菌中的修饰、加工及亚细胞定位

Modification, processing, and subcellular localization in Escherichia coli of the pCloDF13-encoded bacteriocin release protein fused to the mature portion of beta-lactamase.

作者信息

Luirink J, Watanabe T, Wu H C, Stegehuis F, de Graaf F K, Oudega B

出版信息

J Bacteriol. 1987 May;169(5):2245-50. doi: 10.1128/jb.169.5.2245-2250.1987.

DOI:10.1128/jb.169.5.2245-2250.1987
PMID:3553160
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC212142/
Abstract

A fusion between the pCloDF13-derived bacteriocin release protein and beta-lactamase was constructed to investigate the subcellular localization and posttranslational modification of the bacteriocin release protein in Escherichia coli. The signal sequence and 25 of the 28 amino acid residues of the mature bacteriocin release protein were fused to the mature portion of beta-lactamase. The hybrid protein (Mr, 31,588) was expressed in minicells and whole cells and possessed full beta-lactamase activity. Immunoblotting of subcellular fractions revealed that the hybrid protein is present in both the cytoplasmic and outer membranes of E. coli. Radioactive labeling experiments in the presence or absence of globomycin showed that the hybrid protein is modified with a diglyceride and fatty acids and is processed by signal peptidase II, as is the murein lipoprotein. The results indicated that the pCloDF13-encoded bacteriocin release protein is a lipoprotein which is associated with both membranes of E. coli cells.

摘要

构建了来源于pCloDF13的细菌素释放蛋白与β-内酰胺酶的融合体，以研究细菌素释放蛋白在大肠杆菌中的亚细胞定位和翻译后修饰。成熟细菌素释放蛋白的信号序列和28个氨基酸残基中的25个与β-内酰胺酶的成熟部分融合。杂合蛋白（Mr，31,588）在微细胞和全细胞中表达，并具有完整的β-内酰胺酶活性。亚细胞组分的免疫印迹显示杂合蛋白存在于大肠杆菌的细胞质膜和外膜中。在有或没有球霉素的情况下进行的放射性标记实验表明，杂合蛋白如胞壁质脂蛋白一样，被甘油二酯和脂肪酸修饰，并被信号肽酶II加工。结果表明，pCloDF13编码的细菌素释放蛋白是一种脂蛋白，与大肠杆菌细胞的两个膜相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8b2/212142/9fb21feea46f/jbacter00195-0476-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8b2/212142/0a2aafcfd6fc/jbacter00195-0475-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8b2/212142/7902e6fe2112/jbacter00195-0476-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8b2/212142/9fb21feea46f/jbacter00195-0476-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8b2/212142/0a2aafcfd6fc/jbacter00195-0475-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8b2/212142/7902e6fe2112/jbacter00195-0476-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8b2/212142/9fb21feea46f/jbacter00195-0476-b.jpg

相似文献

1
Modification, processing, and subcellular localization in Escherichia coli of the pCloDF13-encoded bacteriocin release protein fused to the mature portion of beta-lactamase.与β-内酰胺酶成熟部分融合的pCloDF13编码的细菌素释放蛋白在大肠杆菌中的修饰、加工及亚细胞定位
J Bacteriol. 1987 May;169(5):2245-50. doi: 10.1128/jb.169.5.2245-2250.1987.
2
A lipoprotein signal peptide plus a cysteine residue at the amino-terminal end of the periplasmic protein beta-lactamase is sufficient for its lipid modification, processing and membrane localization in Escherichia coli.脂蛋白信号肽加上周质蛋白β-内酰胺酶氨基末端的一个半胱氨酸残基，足以使其在大肠杆菌中进行脂质修饰、加工和膜定位。
FEMS Microbiol Lett. 1993 Apr 15;108(3):353-9. doi: 10.1111/j.1574-6968.1993.tb06127.x.
3
Functioning of the stable signal peptide of the pCloDF13-encoded bacteriocin release protein.
Mol Microbiol. 1991 Feb;5(2):393-9. doi: 10.1111/j.1365-2958.1991.tb02121.x.
4
pCloDF13-encoded bacteriocin release proteins with shortened carboxyl-terminal segments are lipid modified and processed and function in release of cloacin DF13 and apparent host cell lysis.编码羧基末端片段缩短的细菌素释放蛋白的pCloDF13经脂质修饰和加工，并在释放cloacin DF13和明显的宿主细胞裂解中发挥作用。
J Bacteriol. 1989 May;171(5):2673-9. doi: 10.1128/jb.171.5.2673-2679.1989.
5
Effect of a mutation preventing lipid modification on localization of the pCloDF13-encoded bacteriocin release protein and on release of cloacin DF13.一种阻止脂质修饰的突变对pCloDF13编码的细菌素释放蛋白定位及cloacin DF13释放的影响。
J Bacteriol. 1988 Sep;170(9):4153-60. doi: 10.1128/jb.170.9.4153-4160.1988.
6
Functioning of a hybrid BRP-beta-lactamase protein in the release of cloacin DF13 and lysis of Escherichia coli cells.
FEMS Microbiol Lett. 1989 Mar;49(1):25-31. doi: 10.1016/0378-1097(89)90336-4.
7
The stable BRP signal peptide causes lethality but is unable to provoke the translocation of cloacin DF13 across the cytoplasmic membrane of Escherichia coli.稳定的芽孢杆菌信号肽会导致致死性，但无法促使绿脓杆菌素DF13穿过大肠杆菌的细胞质膜。
Mol Microbiol. 1992 Aug;6(16):2309-18. doi: 10.1111/j.1365-2958.1992.tb01406.x.
8
Expression of the pCloDF13 encoded bacteriocin release protein or its stable signal peptide causes early effects on protein biosynthesis and Mg2+ transport.
Antonie Van Leeuwenhoek. 1995;67(3):255-60. doi: 10.1007/BF00873689.
9
Structural determinants in addition to the amino-terminal sorting sequence influence membrane localization of Escherichia coli lipoproteins.除氨基末端分选序列外，结构决定因素也影响大肠杆菌脂蛋白的膜定位。
J Bacteriol. 1992 Apr;174(7):2095-101. doi: 10.1128/jb.174.7.2095-2101.1992.
10
Transport and anchoring of beta-lactamase to the external surface of Escherichia coli.β-内酰胺酶向大肠杆菌外表面的转运与锚定
Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2713-7. doi: 10.1073/pnas.89.7.2713.

引用本文的文献

1
Optimization of bacteriocin release protein (BRP)-mediated protein release by Escherichia coli: random mutagenesis of the pCloDF13-derived BRP gene to uncouple lethality and quasi-lysis from protein release.大肠杆菌中细菌素释放蛋白（BRP）介导的蛋白质释放的优化：对源自pCloDF13的BRP基因进行随机诱变，以解除致死性和类溶菌作用与蛋白质释放的关联。
Appl Environ Microbiol. 1998 Feb;64(2):392-8. doi: 10.1128/AEM.64.2.392-398.1998.
2
Escherichia coli SecB, SecA, and SecY proteins are required for expression and membrane insertion of the bacteriocin release protein, a small lipoprotein.大肠杆菌的SecB、SecA和SecY蛋白是细菌素释放蛋白（一种小脂蛋白）表达和膜插入所必需的。
J Bacteriol. 1993 Mar;175(5):1543-7. doi: 10.1128/jb.175.5.1543-1547.1993.
3

本文引用的文献

1
Lipoprotein nature of Bacillus licheniformis membrane penicillinase.地衣芽孢杆菌膜青霉素酶的脂蛋白性质
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3511-5. doi: 10.1073/pnas.78.6.3511.
2
Organization and expression of genes involved in the production of the K88ab antigen.参与K88ab抗原产生的基因的组织与表达
Infect Immun. 1981 Jun;32(3):1155-63. doi: 10.1128/iai.32.3.1155-1163.1981.
3
Comparison of the polypeptide composition of Escherichia coli outer membranes prepared by two methods.两种方法制备的大肠杆菌外膜多肽组成的比较。
The immunity and lysis genes of ColN plasmid pCHAP4.科恩氏菌属质粒pCHAP4的免疫与裂解基因。
Mol Gen Genet. 1988 Feb;211(2):335-41. doi: 10.1007/BF00330613.
4
Effect of a mutation preventing lipid modification on localization of the pCloDF13-encoded bacteriocin release protein and on release of cloacin DF13.一种阻止脂质修饰的突变对pCloDF13编码的细菌素释放蛋白定位及cloacin DF13释放的影响。
J Bacteriol. 1988 Sep;170(9):4153-60. doi: 10.1128/jb.170.9.4153-4160.1988.
5
The acylated precursor form of the colicin A lysis protein is a natural substrate of the DegP protease.大肠杆菌素A裂解蛋白的酰化前体形式是DegP蛋白酶的天然底物。
J Bacteriol. 1989 Nov;171(11):6316-22. doi: 10.1128/jb.171.11.6316-6322.1989.
6
pCloDF13-encoded bacteriocin release proteins with shortened carboxyl-terminal segments are lipid modified and processed and function in release of cloacin DF13 and apparent host cell lysis.编码羧基末端片段缩短的细菌素释放蛋白的pCloDF13经脂质修饰和加工，并在释放cloacin DF13和明显的宿主细胞裂解中发挥作用。
J Bacteriol. 1989 May;171(5):2673-9. doi: 10.1128/jb.171.5.2673-2679.1989.
7
Lipoproteins in bacteria.细菌中的脂蛋白。
J Bioenerg Biomembr. 1990 Jun;22(3):451-71. doi: 10.1007/BF00763177.
8
Structural determinants in addition to the amino-terminal sorting sequence influence membrane localization of Escherichia coli lipoproteins.除氨基末端分选序列外，结构决定因素也影响大肠杆菌脂蛋白的膜定位。
J Bacteriol. 1992 Apr;174(7):2095-101. doi: 10.1128/jb.174.7.2095-2101.1992.
J Bacteriol. 1980 Oct;144(1):425-7. doi: 10.1128/jb.144.1.425-427.1980.
4
Translocation of colicin E1 through cytoplasmic membrane of Escherichia coli.大肠杆菌素E1通过大肠杆菌细胞质膜的转运
FEBS Lett. 1982 Dec 27;150(2):465-8. doi: 10.1016/0014-5793(82)80790-4.
5
The ins and outs of colicins. Part I: Production, and translocation across membranes.大肠杆菌素的来龙去脉。第一部分：产生及跨膜转运。
Microbiol Sci. 1984 Oct;1(7):168-75.
6
Factors necessary for the export process of colicin E1 across cytoplasmic membrane of Escherichia coli.大肠杆菌中大肠杆菌素E1穿过细胞质膜的输出过程所需的因素。
Eur J Biochem. 1984 Apr 16;140(2):249-55. doi: 10.1111/j.1432-1033.1984.tb08095.x.
7
Biosynthesis of Bacillus licheniformis penicillinase in Escherichia coli and in Bacillus subtilis.地衣芽孢杆菌青霉素酶在大肠杆菌和枯草芽孢杆菌中的生物合成。
J Bacteriol. 1983 Nov;156(2):773-7. doi: 10.1128/jb.156.2.773-777.1983.
8
Alternative forms of lethality in mitomycin C-induced bacteria carrying ColE1 plasmids.携带ColE1质粒的丝裂霉素C诱导细菌中的致死性替代形式。
Proc Natl Acad Sci U S A. 1983 Jan;80(2):579-83. doi: 10.1073/pnas.80.2.579.
9
Colicin E2 release: lysis, leakage or secretion? Possible role of a phospholipase.大肠杆菌素E2的释放：裂解、渗漏还是分泌？磷脂酶的可能作用。
EMBO J. 1984 Oct;3(10):2393-7. doi: 10.1002/j.1460-2075.1984.tb02145.x.
10
Studies on the modification and processing of prolipoprotein in Escherichia coli. Effects of structural alterations in prolipoprotein on its maturation in wild type and lpp mutants.大肠杆菌中前脂蛋白修饰与加工的研究。前脂蛋白结构改变对其在野生型和lpp突变体中成熟的影响。
J Biol Chem. 1984 May 25;259(10):6098-104.