Lopez-Talavera J C, Buti M, Casacuberta J, Allende H, Jardi R, Esteban R, Guardia J
Unidad de Hepatologia, Hospital Universitario, Valle de Hebron, Barcelona, Spain.
J Hepatol. 1993 Feb;17(2):199-203. doi: 10.1016/s0168-8278(05)80038-8.
Several studies have demonstrated the presence of serum HDV-RNA by molecular hybridization in patients with chronic D infection, but there is scarce information about the presence of HDV-RNA in hepatic tissue. The presence of HDV-RNA by in situ hybridization (ISH) with a non-radioactive probe in paraffin-embedded hepatic tissue was studied in 29 patients with chronic delta hepatitis (20 with and 9 without intrahepatic delta antigen) and correlate their presence with the expression of hepatic delta antigen and serum HDV-RNA by dot-blot hybridization. HDV-RNA was detected by in situ hybridization in 18 cases: 16 of the 20 (80%) biopsies with intrahepatic delta antigen and 2 of the 9 (22%) without. HDV-RNA was not detected in any of the control cases. Serum HDV-RNA was found in 19 cases: 18 (90%) of 20 chronic delta hepatitis cases with tissular delta antigen and one of the 9 without intrahepatic delta antigen. All patients except one, who was positive for intrahepatic HDV-RNA, showed serum HDV-RNA. However, in two cases ISH detected hepatic HDV-RNA without tissular HDAg; one of these also had serum HDV-RNA and in the other positivity for hepatic HDV-RNA by ISH was the only marker of viral delta replication. In conclusion, detection of HDV-RNA in hepatic tissue by in situ hybridization with a digoxygenin-labelled probe is a rapid and sensitive method that could be a useful tool for diagnosis of HDV infection in clinical laboratories.
多项研究已通过分子杂交证实慢性丁型肝炎感染患者血清中存在丁型肝炎病毒核糖核酸（HDV-RNA），但关于肝组织中HDV-RNA存在情况的信息却很少。我们对29例慢性丁型肝炎患者（20例有肝内丁型抗原，9例无肝内丁型抗原）石蜡包埋肝组织中用非放射性探针通过原位杂交（ISH）检测HDV-RNA的情况进行了研究，并将其存在情况与肝内丁型抗原的表达及通过斑点杂交检测的血清HDV-RNA进行关联分析。通过原位杂交在18例中检测到HDV-RNA：20例有肝内丁型抗原的活检组织中有16例（80%），9例无肝内丁型抗原的活检组织中有2例（22%）。在任何对照病例中均未检测到HDV-RNA。19例中发现血清HDV-RNA：20例有组织丁型抗原的慢性丁型肝炎病例中有18例（90%），9例无肝内丁型抗原的病例中有1例。除1例肝内HDV-RNA阳性患者外，所有患者均显示血清HDV-RNA。然而，在2例中ISH检测到肝内HDV-RNA但无组织丁型肝炎抗原（HDAg）；其中1例也有血清HDV-RNA，另1例通过ISH检测肝内HDV-RNA阳性是病毒丁型复制的唯一标志物。总之，用洋地黄毒苷标记的探针通过原位杂交检测肝组织中的HDV-RNA是一种快速且灵敏的方法，可能成为临床实验室诊断HDV感染的有用工具。
