采用聚合酶链反应（PCR）检测慢性丁型肝炎病毒（HDV）感染患者血清中的HDV-RNA。

Detection of HDV-RNA by PCR in serum of patients with chronic HDV infection.

作者信息

Madejón A, Castillo I, Bartolomé J, Melero M, Campillo M L, Porres J C, Moreno A, Carreño V

机构信息

Department of Gastroenterology, Fundación Jiménez Díaz, Madrid, Spain.

出版信息

J Hepatol. 1990 Nov;11(3):381-4. doi: 10.1016/0168-8278(90)90225-g.

DOI:10.1016/0168-8278(90)90225-g

PMID:2290030

Abstract

In this paper, we studied the usefulness of polymerase chain reaction (PCR) in HDV-RNA detection. Using serial dilutions of serum samples of known concentrations of HDV-RNA, PCR was 10,000-times more sensitive than slot-blot hybridization. PCR was used for the detection of HDV-RNA in 33 serum samples negative to HDV-RNA by conventional slot-blot hybridization. HDV-RNA was detected in 18/33 (54%) of the samples included in this study using PCR. When positivity to a viral genome was related to other viral replication markers, it was found that among the 18 patients positive to the viral genome, 13 (72%) had hepatitis delta antigen in the liver, and five (28%) were negative. In conclusion, HDV-RNA detection by gene amplification is 10,000-times more sensitive than slot-blot hybridization, and allows the detection of viral replication in patients without other viral replication markers.

摘要

在本文中，我们研究了聚合酶链反应（PCR）在丁型肝炎病毒RNA（HDV-RNA）检测中的实用性。通过对已知HDV-RNA浓度的血清样本进行系列稀释，PCR的灵敏度比斑点杂交高10000倍。PCR用于检测33份经传统斑点杂交检测HDV-RNA呈阴性的血清样本中的HDV-RNA。在本研究纳入的样本中，使用PCR在18/33（54%）的样本中检测到了HDV-RNA。当病毒基因组阳性与其他病毒复制标志物相关时，发现在18例病毒基因组阳性的患者中，13例（72%）肝脏中有丁型肝炎抗原，5例（28%）为阴性。总之，通过基因扩增检测HDV-RNA的灵敏度比斑点杂交高10000倍，并且能够在没有其他病毒复制标志物的患者中检测到病毒复制。