Hofer M, Pospísil M, Viklická S, Vacek A, Pipalová I, Bartonícková A
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno.
J Leukoc Biol. 1993 Feb;53(2):185-9. doi: 10.1002/jlb.53.2.185.
A combination of diclofenac and glucan administered repeatedly in a protective regimen in the course of repeated gamma irradiation of mice (6 x 2 Gy during 3 weeks) enhanced granulopoiesis and other indices of hematopoietic recovery investigated from 3 to 7 days after the last radiation exposure. Repeated administration of diclofenac or glucan alone or treatment of the mice with the diclofenac-glucan combination given once before the first or the last radiation exposure did not induce such effects. The protective effect of the repeatedly administered combination of the drugs was realized despite the fact that the response of the serum colony-stimulating activity to the repeated combined drug administration was decreased at the end of the treatment regimen compared to that of mice given this drug combination only once. The combined treatment is supposed to act via increased proliferation of the hematopoietic stem or progenitor cells. Additivity or even synergism of the hematostimulatory action of glucan and of the strengthening of positive control of cell proliferation achieved by removing negatively acting prostaglandins (diclofenac action) may account for the radioprotective effects observed.
在对小鼠进行重复γ射线照射（3周内6次，每次2 Gy）的过程中，以保护方案反复给予双氯芬酸和葡聚糖，可增强粒细胞生成以及在最后一次辐射暴露后3至7天所研究的其他造血恢复指标。单独反复给予双氯芬酸或葡聚糖，或在第一次或最后一次辐射暴露前仅给予一次双氯芬酸 - 葡聚糖组合对小鼠进行治疗，均未产生此类效果。尽管与仅给予一次该药物组合的小鼠相比，在治疗方案结束时血清集落刺激活性对反复联合给药的反应有所降低，但反复给予药物组合的保护作用仍得以实现。联合治疗被认为是通过增加造血干细胞或祖细胞的增殖来发挥作用的。葡聚糖的造血刺激作用与通过去除负性作用的前列腺素（双氯芬酸的作用）实现的细胞增殖正调控增强之间的相加甚至协同作用，可能是观察到的辐射防护效果的原因。
