通过数字压缩在五个通道中对两个DNA模板进行测序。

Sequencing two DNA templates in five channels by digital compression.

作者信息

Nelson M, Zhang Y, Steffens D L, Grabherr R, Van Etten J L

机构信息

Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.

出版信息

Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1647-51. doi: 10.1073/pnas.90.5.1647.

DOI:10.1073/pnas.90.5.1647

PMID:8446578

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC45936/

Abstract

By applying algebraic coding methods to the Sanger dideoxynucleotide procedure, DNA sequences of two templates can be determined simultaneously in only five reactions and data channels. A 5:2 data compression is accomplished by instantaneous source coding of nucleotide sequence pairs into one set of 5-bit block codes. A general algebraic expression, 2n-1 > or = 4f, describes conditions under which f DNA templates can be sequenced using n channels. Such compression sequencing is accurate and efficient, as demonstrated by manual 35S autoradiographic detection and automated on-line analysis using fluorescent-labeled primers. Symmetric 5:2 compression is especially useful when comparing two closely related sequences.

摘要

通过将代数编码方法应用于桑格双脱氧核苷酸测序法，仅需五个反应和数据通道就能同时测定两个模板的DNA序列。通过将核苷酸序列对即时源编码为一组5位分组码，实现了5:2的数据压缩。一个通用的代数表达式2n - 1≥4f描述了使用n个通道对f个DNA模板进行测序的条件。如通过手动35S放射自显影检测以及使用荧光标记引物的自动在线分析所证明的，这种压缩测序准确且高效。当比较两个密切相关的序列时，对称5:2压缩尤其有用。