Wolf C, Rouyer N, Lutz Y, Adida C, Loriot M, Bellocq J P, Chambon P, Basset P
Laboratoire de Génétique Moléculaire des Eucaryotes, Centre National de la Recherche Scientifique, Strasbourg, France.
Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1843-7. doi: 10.1073/pnas.90.5.1843.
The expression of the stromelysin 3 (ST3) gene, which encodes a putative matrix metalloproteinase, was studied during breast cancer progression. The ST3 gene is expressed in all invasive breast carcinomas, in a number of their metastases, and in some in situ carcinomas where the probability of detecting ST3 transcripts correlates with the known risk of these carcinomas to become invasive. ST3 RNA and protein were specifically detected in fibroblastic cells immediately surrounding the neoplastic cells in both primary and metastatic tumors. This expression pattern distinguishes the ST3 gene from other matrix metalloproteinase genes, most notably from the 72-kDa type IV collagenase gene, which can be expressed in fibroblastic cells distributed throughout the stroma of primary breast carcinomas. Furthermore, high levels of 72-kDa type IV collagenase, but not of ST3 transcripts, are detected in benign breast fibroadenomas. Interestingly, the urokinase and ST3 genes exhibit very similar patterns of expression in breast carcinomas, which suggests that their products may cooperate during cancer progression.
对编码一种假定基质金属蛋白酶的基质溶解素3（ST3）基因在乳腺癌进展过程中的表达进行了研究。ST3基因在所有浸润性乳腺癌、其一些转移灶以及一些原位癌中表达，在这些原位癌中检测到ST3转录本的概率与这些癌变为浸润性癌的已知风险相关。在原发性和转移性肿瘤中紧邻肿瘤细胞的成纤维细胞中特异性检测到了ST3 RNA和蛋白质。这种表达模式将ST3基因与其他基质金属蛋白酶基因区分开来，最显著的是与72 kDa IV型胶原酶基因不同，后者可在分布于原发性乳腺癌基质中的成纤维细胞中表达。此外，在良性乳腺纤维腺瘤中检测到高水平的72 kDa IV型胶原酶，但未检测到ST3转录本。有趣的是，尿激酶和ST3基因在乳腺癌中表现出非常相似的表达模式，这表明它们的产物在癌症进展过程中可能协同作用。
