Engel G, Heselmeyer K, Auer G, Bäckdahl M, Eriksson E, Linder S
Department of General Oncology, Radiumhemmet, Karolinska Institute and Hospital, Stockholm, Sweden.
Int J Cancer. 1994 Sep 15;58(6):830-5. doi: 10.1002/ijc.2910580614.
The expression level of stromelysin-3 (ST3) mRNA was analyzed by in situ hybridization of formalin-fixed, paraffin-embedded primary breast-tumor samples from 76 patients. Digital image analysis of the dark-field in situ hybridization signal was used to measure the maximal level of ST3 expression in each tumor. All 55 invasive ductal carcinomas and 9 of 10 invasive lobular carcinomas were positive for ST3. Invasive tumors had significantly higher levels of ST3 than in situ tumors. Furthermore, ST3 levels were higher in invasive ductal carcinomas than in invasive lobular carcinomas. The ST3 expression level was significantly correlated to fatal metastatic disease (mean follow-up 104 months). ST3 levels of < 2,500 units were associated with distant metastasis in 46% of patients, whereas levels of > 2,500 units were associated with metastasis in 79% of patients selected for study. ST3 mRNA levels did not correlate with tumor size, microvessel density, DNA ploidy or estrogen-receptor levels. Studies of ST3 expression may provide information valuable for the understanding of breast cancer biology and for prognosis.
通过对76例患者福尔马林固定、石蜡包埋的原发性乳腺肿瘤样本进行原位杂交，分析基质溶解素-3（ST3）mRNA的表达水平。利用暗视野原位杂交信号的数字图像分析来测量每个肿瘤中ST3表达的最大水平。所有55例浸润性导管癌和10例浸润性小叶癌中的9例ST3呈阳性。浸润性肿瘤的ST3水平显著高于原位肿瘤。此外，浸润性导管癌中的ST3水平高于浸润性小叶癌。ST3表达水平与致命性转移性疾病显著相关（平均随访104个月）。ST3水平<2500单位的患者中46%发生远处转移，而在入选研究的患者中，ST3水平>2500单位的患者中有79%发生转移。ST3 mRNA水平与肿瘤大小、微血管密度、DNA倍体或雌激素受体水平无关。对ST3表达的研究可能为理解乳腺癌生物学特性和预后提供有价值的信息。
