Köhler H, Nustede R, Lüdtke F E, Barthel M, Schlemminger R, Schafmayer A
Department of General Surgery, University of Göttingen, Federal Republic of Germany.
Res Exp Med (Berl). 1993;193(1):47-56. doi: 10.1007/BF02576210.
Besides vagal cholinergic mechanisms, pancreatic polypeptide (PP) secretion is thought to be mediated by hormones. This study was performed to delineate the role of extrinsic pancreatic innervation and cholecystokinin (CCK) in amino acid- and fat-stimulated PP secretion. In ten mongrel dogs, pancreatic denervation was performed by the method of Debas et al. [3]. Total denervation of the pancreas did not alter PP response to intraduodenal application of amino acids (integrated output 24,434 +/- 3260 pmol/1 x 120 min before vs 22,797 +/- 2470 pmol/1 x 120 min after operation) and to intraduodenal fat solution (19,595 +/- 2121 pmol/1 x 120 min vs 19,983 +/- 2031 pmol/1 x 120 min). Also, no significant differences were measured in CCK release (491 +/- 71 pmol/1 x 120 min vs 430 +/- 57 pmol/1 x 120 min for amino acids, 571 = 63 pmol/1 x 120 min vs 563 +/- 89 pmol/1 x 120 min for fat solution). Plasma PP and CCK levels were compared by linear regression analysis. Correlations between PP and CCK were high in the intact pancreas (amino acids, r = 0.92; fat, r = 0.99) as well as in the denervated pancreas (r = 0.93 amino acids and r = 0.98 fat). These results show that extrinsic pancreatic innervation does not influence PP and CCK release after intraduodenal amino acids or fat solution and that PP secretion seems to be mediated to some extent through the release of CCK.
除了迷走胆碱能机制外，胰多肽（PP）的分泌被认为是由激素介导的。本研究旨在阐明胰腺外神经支配和胆囊收缩素（CCK）在氨基酸和脂肪刺激的PP分泌中的作用。在10只杂种狗中，采用德巴斯等人[3]的方法进行胰腺去神经支配。胰腺完全去神经支配并未改变PP对十二指肠内应用氨基酸（手术前120分钟内综合输出为24,434±3260 pmol/1，手术后为22,797±2470 pmol/1×120分钟）和十二指肠内脂肪溶液（19,595±2121 pmol/1×120分钟对19,983±2031 pmol/1×120分钟）的反应。此外，在CCK释放方面也未测得显著差异（氨基酸刺激时为491±71 pmol/1×120分钟对430±57 pmol/1×120分钟，脂肪溶液刺激时为571±63 pmol/1×120分钟对563±89 pmol/1×120分钟）。通过线性回归分析比较血浆PP和CCK水平。在完整胰腺中（氨基酸，r = 0.92；脂肪，r = 0.99）以及去神经支配的胰腺中（氨基酸，r = 0.93；脂肪，r = 0.98），PP和CCK之间的相关性都很高。这些结果表明，胰腺外神经支配不影响十二指肠内氨基酸或脂肪溶液后PP和CCK的释放，并且PP分泌似乎在一定程度上是通过CCK的释放介导的。
