粗糙脉孢菌复合体I的核编码10.5 kDa亚基的一级结构。
Primary structure of the nuclear-encoded 10.5 kDa subunit of complex I from Neurospora crassa.
作者信息
Duarte M, Belo J A, Videira A
机构信息
Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Portugal.
出版信息
Biochim Biophys Acta. 1993 Mar 20;1172(3):327-8. doi: 10.1016/0167-4781(93)90223-z.
PMID:8448210
Abstract
We have isolated a cDNA clone for the nuclear encoded 10.5 kDa subunit of complex I from N. crassa. DNA sequencing revealed an open reading frame corresponding to a polypeptide with 94 amino acids and a calculated molecular mass of 10531 Da. The protein is synthesized without a cleavable mitochondrial targeting sequence. The N. crassa polypeptide is the fungal equivalent of subunit B8 of bovine complex I.
摘要
我们从粗糙脉孢菌中分离出了编码复合体I核基因10.5 kDa亚基的cDNA克隆。DNA测序显示一个开放阅读框，对应一个含94个氨基酸、计算分子量为10531 Da的多肽。该蛋白质合成时没有可切割的线粒体靶向序列。粗糙脉孢菌的这个多肽相当于牛复合体I的B8亚基。
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