Verdera S, Leòn A, Kronauge J F, Leòn E, Souto B, Oliver P, Rodriguez A, Tamosiunas G, Esponda A, Jones A G
Departamento de Radiofarmacia, Centro de Investigaciones Nucleares, Fac. de Ciencias, Montevideo, Uruguay.
Nucl Med Biol. 1993 Feb;20(2):127-32. doi: 10.1016/0969-8051(93)90104-3.
Extraction, washout and chemical integrity of technetium(I) hexakis (2-carbomethoxy-2-isocyano propane), (99mTc-CPI) were evaluated in isolated contractile rat atrial tissue. 99mTc-CPI accumulated linearly over time at 32 degrees C with uptakes that were both concentration dependent and temperature sensitive. Uptake rates also increased with contractile strength, indicating a dependence of retention on metabolic status of the tissue. Retention indices showed that percent washout of 99mTc-CPI was much slower than control studies with 99mTcO4. HPLC analysis of retained radioactive products in atrial tissue showed most of the activity present as the 99mTc-CPI complex (75%). These observations in isolated rat atrial tissue help explain the interspecies differences in biodistribution of 99mTc-CPI and support the membrane potential dependent model for uptake and retention of technetium isonitrile complexes.
在离体收缩的大鼠心房组织中评估了六（2-甲氧羰基-2-异氰基丙烷）锝（I）（99mTc-CPI）的提取、洗脱和化学完整性。99mTc-CPI在32℃下随时间呈线性积累，摄取既依赖浓度又对温度敏感。摄取率也随收缩强度增加，表明保留依赖于组织的代谢状态。保留指数显示，99mTc-CPI的洗脱百分比比用99mTcO4进行的对照研究慢得多。对心房组织中保留的放射性产物进行的HPLC分析表明，大部分活性以99mTc-CPI复合物的形式存在（75%）。在离体大鼠心房组织中的这些观察结果有助于解释99mTc-CPI生物分布的种间差异，并支持锝异腈络合物摄取和保留的膜电位依赖性模型。
