Vuagnat P
Immunology. 1977 Jan;32(1):103-9.
The capacity of non-reciprocally contaminated guinea-pig IgG1 and IgG2 antibodies for sensitizing antigen-coated sheep erythrocytes for lysis was studied in the presence of serial dilutions of normal guinea-pig serum as the source of complement. IgG1 antibodies were highly efficient, provided complement was little diluted and not heated at 56 degrees. Chelation of Ca2+ by EGTA affected lysis of IgG1-sensitized cells by only 50%, while completely blocking lysis of IgG2-sensitized cells. The F(ab')2 fragment of IgG1, in contrast to the F(ab')2 fragment of IgG2, was almost as efficient as the parent antibody.
在以系列稀释的正常豚鼠血清作为补体来源的情况下，研究了非相互污染的豚鼠IgG1和IgG2抗体致敏抗原包被的绵羊红细胞以进行裂解的能力。如果补体稀释程度小且未在56摄氏度加热，则IgG1抗体效率很高。EGTA对Ca2+的螯合仅使IgG1致敏细胞的裂解受到50%的影响，而完全阻断了IgG2致敏细胞的裂解。与IgG2的F(ab')2片段相比，IgG1的F(ab')2片段几乎与亲本抗体一样有效。
