Klaus G G, Pepys M B, Kitajima K, Askonas B A
Immunology. 1979 Dec;38(4):687-95.
The capacity of mouse IgM, IgGl, IgG2 and IgA anti-dinitrophenyl (DNP) antibodies to activate mouse or guinea-pig complement was studied, using a sensitive haemolytic assay and two-dimensional immunoelectrophoresis to detect cleavage of mouse C3. Three monoclonal IgM antibodies, and a heterogeneous IgM fraction, lysed trinitrophenylated erythrocytes in the presence of guinea-pig C, but failed to produce lysis in the presence of mouse C. and only activated mouse C3 very inefficiently. A monoclonal IgGl antibody did not produce haemolysis in the presence of guinea-pig or mouse C, but cleaved mouse C3 via the alternative pathway. Two IgA myeloma proteins (M315 and M460) had similar properties. A heterogeneous IgG2 antibody fraction produced haemolysis in the presence of both mouse and guinea-pig C, and was shown to activate both the classical and alternative pathways of mouse C.
利用灵敏的溶血试验和二维免疫电泳检测小鼠C3的裂解情况，研究了小鼠IgM、IgG1、IgG2和IgA抗二硝基苯基（DNP）抗体激活小鼠或豚鼠补体的能力。三种单克隆IgM抗体和一种异质性IgM组分在豚鼠补体存在的情况下能使三硝基苯基化红细胞溶解，但在小鼠补体存在时未能产生溶解现象，并且对小鼠C3的激活效率非常低。一种单克隆IgG1抗体在豚鼠或小鼠补体存在时均未产生溶血现象，但可通过替代途径裂解小鼠C3。两种IgA骨髓瘤蛋白（M315和M460）具有相似的特性。一种异质性IgG2抗体组分在小鼠和豚鼠补体存在时均能产生溶血现象，并且显示出可激活小鼠补体的经典途径和替代途径。
