Horvat B, Damjanov I
Department of Pathology and Cell Biology, Thomas Jefferson University, Jefferson Medical College, Philadelphia, Pa. 19107.
Gynecol Obstet Invest. 1993;35(2):69-75. doi: 10.1159/000292668.
The synthesis of cellular proteins during early pregnancy was analyzed in mouse endometria incubated in vitro in the presence of [35S]-methionine. Endometrial proteins labelled on days 3 and 5 of pregnancy were compared with proteins from nonpregnant proestrus-phase endometrium. We have identified 23 proteins that were differentially expressed. These proteins were divided into four groups: (1) 10 proteins synthesized most prominently in nonpregnant proestrus-phase endometrium that decrease or disappear in pregnancy; (2) 4 proteins that are synthesized only on day 3, i.e., in the immediate preimplantation period; (3) 3 proteins that appear only on day 5 coincidentally with the development of decidua; (4) 5 proteins that are synthesized by both day 3 and day 5 endometria but are not found in the nonpregnant endometrium. Hence, the synthesis of some endometrial cellular proteins increased and others decreased in early pregnancy. The function of these proteins is unknown. Four proteins (Mr 26, 51, 52 and 70 kDa), 2 of which are both cell associated and secreted (Mr 52 and 70 kd) were selectively synthesized in the preimplantation period and thus may mediate implantation.
在存在[35S]-甲硫氨酸的情况下，对体外培养的小鼠子宫内膜在妊娠早期的细胞蛋白质合成进行了分析。将妊娠第3天和第5天标记的子宫内膜蛋白与未妊娠动情前期子宫内膜的蛋白进行比较。我们已经鉴定出23种差异表达的蛋白质。这些蛋白质被分为四组：（1）10种在未妊娠动情前期子宫内膜中最显著合成的蛋白质，在妊娠时减少或消失；（2）4种仅在第3天，即在植入前期合成的蛋白质；（3）3种仅在第5天与蜕膜发育同时出现的蛋白质；（4）5种在第3天和第5天的子宫内膜中均有合成但在未妊娠子宫内膜中未发现的蛋白质。因此，在妊娠早期，一些子宫内膜细胞蛋白质的合成增加，而另一些则减少。这些蛋白质的功能尚不清楚。四种蛋白质（分子量分别为26、51、52和70 kDa），其中两种既与细胞相关又可分泌（分子量为52和70 kd）在植入前期被选择性合成，因此可能介导着床。
