染色体重新定位：同时包含YES1和胸苷酸合成酶的酵母人工染色体定位于18号染色体短臂。
Chromosomal reassignment: YACs containing both YES1 and thymidylate synthase map to the short arm of chromosome 18.
作者信息
Silverman G A, Kuo W L, Taillon-Miller P, Gray J W
机构信息
Department of Pediatrics, Joint Program in Neonatology, Harvard Medical School, Boston, Massachusetts 02115.
出版信息
Genomics. 1993 Feb;15(2):442-5. doi: 10.1006/geno.1993.1086.
The YES1 proto-oncogene was mapped previously to human chromosome band 18q21.3 by using isotopic in situ hybridization. Using yeast artificial chromosomes (YACs) as probes and fluorescence in situ hybridization, a strong signal was detected in the region corresponding to 18p11.3. Restriction digests confirmed that the YACs contained the YES1 gene and not other cross-hybridizing, protein-tyrosine kinases. In addition, these YACs were found to contain another 18p11.32 gene, thymidylate synthase. These genes were less than 50 kb apart. Collectively, these data suggest that YES1 maps to 18p11.32 rather than to 18q21.3.
先前通过同位素原位杂交将YES1原癌基因定位于人类染色体18q21.3带。使用酵母人工染色体（YAC）作为探针并进行荧光原位杂交，在对应于18p11.3的区域检测到强信号。限制性酶切证实这些YAC包含YES1基因而非其他交叉杂交的蛋白酪氨酸激酶。此外，发现这些YAC还包含另一个18p11.32基因，胸苷酸合成酶。这些基因相距不到50 kb。总体而言，这些数据表明YES1定位于18p11.32而非18q21.3。
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