Das K M, Dasgupta A, Mandal A, Geng X
Department of Medicine, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick 08903.
J Immunol. 1993 Mar 15;150(6):2487-93.
Autoimmunity has been emphasized in the pathogenesis of ulcerative colitis and various anticolon antibodies have been described. Nevertheless, the nature of the autoantigens has been elusive. Using ulcerative colitis colon-eluted IgG antibody, we earlier detected an M(r) 40,000 protein, P40, in colon extract. In our study, we purified P40 from human colon to apparent homogeneity, sequenced two peptides after proteolytic digestion, and demonstrated that the P40 belongs to the tropomyosin family. Furthermore, we demonstrated that blood serum from 95% of patients with ulcerative colitis contains antibodies that are reactive to tropomyosin.
自身免疫在溃疡性结肠炎的发病机制中已受到重视，并且已描述了多种抗结肠抗体。然而，自身抗原的性质一直难以捉摸。我们早期利用溃疡性结肠炎结肠洗脱的IgG抗体，在结肠提取物中检测到一种分子量为40,000的蛋白质P40。在本研究中，我们从人结肠中纯化P40至明显的均一性，对其进行蛋白水解消化后测序了两个肽段，并证明P40属于原肌球蛋白家族。此外，我们还证明95%的溃疡性结肠炎患者血清中含有对原肌球蛋白有反应的抗体。
