变形链球菌GS-5的染色体组织

Chromosome organization of Streptococcus mutans GS-5.

作者信息

Hantman M J, Sun S, Piggot P J, Daneo-Moore L

机构信息

Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.

出版信息

J Gen Microbiol. 1993 Jan;139(1):67-77. doi: 10.1099/00221287-139-1-67.

DOI:10.1099/00221287-139-1-67

PMID:8450309

Abstract

Twenty-eight genetic loci have been physically mapped to specific large restriction fragments of the Streptococcus mutans GS-5 chromosome by hybridization with probes of cloned genes or, for transposon-generated amino acid auxotrophs, with probes for Tn916. In addition, restriction fragments generated by one low-frequency-cleavage enzyme were used as probes to identify overlapping fragments generated by other restriction enzymes. The approach allowed construction of a low resolution physical map of the S. mutans GS-5 genome using restriction enzymes ApaI (5'-GGGCC/C), SmaI (5'-CCC/GGG), and NotI (5'-GC/GGCCGC).

摘要

通过与克隆基因的探针杂交，或者对于转座子产生的氨基酸营养缺陷型，与Tn916的探针杂交，已将28个基因位点物理定位到变形链球菌GS-5染色体的特定大限制性片段上。此外，由一种低频切割酶产生的限制性片段被用作探针，以鉴定由其他限制性酶产生的重叠片段。该方法允许使用限制性酶ApaI（5'-GGGCC/C）、SmaI（5'-CCC/GGG）和NotI（5'-GC/GGCCGC）构建变形链球菌GS-5基因组的低分辨率物理图谱。