化脓性链球菌M1型菌株的物理和遗传染色体图谱。
Physical and genetic chromosomal map of an M type 1 strain of Streptococcus pyogenes.
作者信息
Suvorov A N, Ferretti J J
机构信息
Institute of Experimental Medicine, Pavlova, St. Petersburg, Russia.
出版信息
J Bacteriol. 1996 Sep;178(18):5546-9. doi: 10.1128/jb.178.18.5546-5549.1996.
Abstract
A physical map of the chromosome of an M type 1 strain of Streptococcus pyogenes was constructed following digestion with three different restriction enzymes, SmaI, SfiI, and SgrAI, and separation and analysis of fragments by pulsed-field gel electrophoresis. The genome size of this strain was estimated to be 1,920 kb. By employing Southern hybridization and PCR analysis, 36 genes were located on the map.
摘要
用三种不同的限制性内切酶SmaI、SfiI和SgrAI消化化脓性链球菌M1型菌株的染色体，然后通过脉冲场凝胶电泳分离和分析片段，构建了该菌株的物理图谱。该菌株的基因组大小估计为1920 kb。通过Southern杂交和PCR分析，在图谱上定位了36个基因。
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