Shinoda S, Okamoto K
J Bacteriol. 1977 Mar;129(3):1266-71. doi: 10.1128/jb.129.3.1266-1271.1977.
Formation of the lateral flagella (L-flagella) of Vibrio parahaemolyticus was studied immunologically, using specific antiserum against L-flagella. On solid medium, L-flagella were formed at both high (37 degrees C) and low (25 degrees C) temperatures, although at high temperatures they became dissociated from the cells and decomposed in the medium. L-flagella were not formed in liquid or soft-agar medium. Formation of L-flagella was decreased by lowering the pH of the medium and repressed by transferring the cells from solid medium to liquid medium. Mutants possessing L-flagella but not a polar monotrichous flagellum (M-flagellum) swarmed on solid medium, whereas mutants were grown on solid medium and then transfered to liquid medium, the cells oscillated until they lost L-flagella. It is postulated that L-flagella are locomotive organelles on solid medium and in some cases also in liquid medium, whereas M-flagella are locomotive organelles only in liquid medium.
利用抗副溶血弧菌侧鞭毛（L-鞭毛）的特异性抗血清，对副溶血弧菌L-鞭毛的形成进行了免疫学研究。在固体培养基上，L-鞭毛在高温（37℃）和低温（25℃）下均能形成，不过在高温下它们会从细胞上解离并在培养基中分解。在液体或软琼脂培养基中不形成L-鞭毛。降低培养基的pH值会减少L-鞭毛的形成，将细胞从固体培养基转移到液体培养基会抑制L-鞭毛的形成。具有L-鞭毛但没有极生单鞭毛（M-鞭毛）的突变体在固体培养基上会成群游动，而将突变体在固体培养基上培养后再转移到液体培养基中，细胞会摆动直至失去L-鞭毛。据推测，L-鞭毛是固体培养基上以及某些情况下在液体培养基中的运动细胞器，而M-鞭毛仅是液体培养基中的运动细胞器。
