Selstam G, Nilsson I, Mattsson M O
Department of Physiology, University of Umeå, Sweden.
Acta Physiol Scand. 1993 Jan;147(1):123-9. doi: 10.1111/j.1748-1716.1993.tb09480.x.
The occurrence of the intermediate filament desmin in ovary and corpus luteum of pseudopregnant rats was studied using Western blot analysis and immunohistochemistry. The luteal phase was induced by mating with vasectomized male rats and ovaries were studied after 6, 11 and 19 days. The findings from the Western blot analysis showed that desmin was present in the corpus luteum. Immunohistochemical localization of desmin showed two types of localization in the corpus luteum. The arteries around the corpus luteum, as well as arteries elsewhere in the ovary, had a high content of desmin in their muscle layer. Dispersed in the corpus luteum was an immunohistochemical staining of desmin that was localized mainly adjacent to the luteal cells. In the other part of the ovary a weak staining was registered in the theca layer, no staining in the granulosa layer and a streaky staining in the hilar region of the ovary. Desmin filaments are found in muscle cells of all types, including vascular smooth muscle cells. Probably, all desmin in the ovary is localized to smooth muscle cells with the possible exception of the corpus luteum where very few muscle cells have been identified. Localization to other vascular cells as endothelial is possible. In this study we found an increase in desmin content in the corpus luteum after day 6. If desmin is related to vascular resistance, our finding is consistent with the decrease in blood flow that occurs after day 6.
运用蛋白质免疫印迹分析和免疫组织化学方法，研究中间丝结蛋白在假孕大鼠卵巢和黄体中的表达情况。通过与输精管结扎的雄性大鼠交配诱导黄体期，并在6天、11天和19天后对卵巢进行研究。蛋白质免疫印迹分析结果显示，黄体中存在结蛋白。结蛋白的免疫组织化学定位显示，黄体中有两种定位类型。黄体周围的动脉以及卵巢其他部位的动脉，其肌层中结蛋白含量较高。分散在黄体中的结蛋白免疫组织化学染色主要定位于黄体细胞附近。在卵巢的其他部分，在卵泡膜层有弱阳性染色，颗粒层无染色，卵巢门区有条纹状染色。结蛋白丝存在于所有类型的肌肉细胞中，包括血管平滑肌细胞。卵巢中的所有结蛋白可能都定位于平滑肌细胞，黄体可能是例外，因为在黄体中仅发现极少数肌肉细胞。结蛋白也可能定位于其他血管细胞，如内皮细胞。在本研究中，我们发现6天后黄体中的结蛋白含量增加。如果结蛋白与血管阻力有关，我们的发现与6天后出现的血流量减少是一致的。
