Nilsson I, Mattsson M O, Selstam G
Department of Physiology, University of Umeå, Sweden.
Histochem Cell Biol. 1995 Mar;103(3):237-42. doi: 10.1007/BF01454029.
The presence of the intermediate filament (IF) proteins cytokeratins and vimentin in corpus luteum (CL) and other parts of the ovary from adult pseudopregnant rats was investigated using immunohistochemistry and immunoblot analysis. To induce pseudopregnancy, female rats were mated with sterile male rats. The mating procedure induces a prolonged luteal life-span of 13 +/- 1 days. Positive staining for cytokeratin could be seen in CL, in the theca layer of follicles, and the ovarian surface epithelium with the broad-spectrum monoclonal antibody cocktail AE1/AE3. Weak staining was also seen in CL with antibodies against cytokeratins 8 and 18. A similar distribution was also seen for vimentin, which furthermore was detected in blood vessels. No changes in staining intensity was seen in CL of different luteal age. The strong staining for vimentin in CL was confirmed by immunoblot analysis, where one main band of 57 kDa was observed from day 1 to day 19 of pseudopregnancy. Expression of the IF proteins investigated seems to start in the newly formed CL and the continuous expression indicates that they are not directly regulated by luteal steroids.
利用免疫组织化学和免疫印迹分析，研究了成年假孕大鼠黄体（CL）及卵巢其他部位中间丝（IF）蛋白细胞角蛋白和波形蛋白的存在情况。为诱导假孕，将雌性大鼠与不育雄性大鼠交配。交配过程可诱导黄体寿命延长至13±1天。使用广谱单克隆抗体混合物AE1/AE3，可在黄体、卵泡膜层和卵巢表面上皮中观察到细胞角蛋白的阳性染色。用抗细胞角蛋白8和18的抗体在黄体中也可见弱阳性染色。波形蛋白也有类似分布，此外在血管中也可检测到。不同黄体期的黄体染色强度未见变化。免疫印迹分析证实了黄体中波形蛋白的强染色，在假孕第1天至第19天观察到一条57 kDa的主要条带。所研究的中间丝蛋白的表达似乎始于新形成的黄体，持续表达表明它们不受黄体类固醇的直接调节。
