Mooney M P, Losken H W, Tschakaloff A, Siegel M I, Losken A, Lalikos J F
Department of Anatomy and Histology, University of Pittsburgh, Pennsylvania 15261.
Cleft Palate Craniofac J. 1993 Mar;30(2):121-8. doi: 10.1597/1545-1569_1993_030_0121_cbcssi_2.3.co_2.
Experimental rabbit models of postnatal coronal suture (CS) synostosis have helped make significant contributions towards the understanding and surgical management of human congenital craniosynostosis. The present study compares craniofacial growth patterns in animals with experimental CS immobilization and in a rabbit born in our laboratory with congenital CS synostosis. The study sample consisted of 10 sham controls, 14 experimental animals with bilateral CS immobilization, and one animal with congenital, bilateral CS synostosis. At 1.5 weeks of age, all animals had amalgam markers placed on either side of the frontonasal, coronal, and anterior lambdoid sutures. At this time, the experimental animals had bilateral CS immobilization using methyl-methacrylate. Serial lateral head x-rays were taken at 1.5, 6, 12, and 18 weeks of age. Results revealed that by 1.5 weeks of age the congenital animal already exhibited changes in the cranial vault, cranial base, midface, and orthocephalic cranial base angles compared to controls. By 6 weeks of age, animals with experimental immobilization showed compensatory growth patterns similar to the congenital animal, particularly at the calvarial sutures and upper midface. This pattern continued through 18 weeks. Results showed that experimental, postnatal CS immobilization produced similar craniofacial growth patterns to those observed for our single congenital animal, but to a lesser degree, and therefore validates, in part, findings from experimental rabbit models of synostosis.
出生后冠状缝（CS）早闭的实验性兔模型有助于对人类先天性颅缝早闭的理解和手术治疗做出重大贡献。本研究比较了实验性CS固定动物和我们实验室出生的患有先天性CS早闭的兔子的颅面生长模式。研究样本包括10只假手术对照组、14只双侧CS固定的实验动物和1只患有先天性双侧CS早闭的动物。在1.5周龄时，所有动物在前额鼻缝、冠状缝和前人字缝两侧放置汞合金标记物。此时，对实验动物使用甲基丙烯酸甲酯进行双侧CS固定。在1.5、6、12和18周龄时拍摄系列头颅侧位X线片。结果显示，与对照组相比，到1.5周龄时，先天性动物在颅顶、颅底、中面部和正颅底角已经出现变化。到6周龄时，实验性固定的动物表现出与先天性动物相似的代偿性生长模式，特别是在颅盖缝和上颌中部。这种模式持续到18周。结果表明，实验性出生后CS固定产生的颅面生长模式与我们的单一先天性动物所观察到的相似，但程度较轻，因此部分验证了缝早闭实验性兔模型的研究结果。
