Sallaz M, Jourdan F
CNRS, Université Claude Bernard/Lyon, Villeurbanne, France.
Neuroreport. 1993 Jan;4(1):55-8. doi: 10.1097/00001756-199301000-00014.
Using a selective monoclonal Fos antibody, we have studied the expression of the c-fos proto-oncogene in the olfactory bulb of awake rats stimulated with propionic acid vapours. We have perfected a method which allows the comparison of patterns of either cellular c-fos immunoreactivity or glomerular 2-deoxyglucose uptake in the same olfactory bulb. C-fos was expressed in local bulbar interneurones, principally granule cells. We observed that the low level of basal c-fos expression raised significantly under olfactory stimulation in the bulbar column defined by the foci of high 2-deoxyglucose glomerular uptake. This is the first demonstration that c-fos expression can be triggered by afferent olfactory input in the olfactory bulb in normally breathing awake rats. These data support the assumption that the olfactory bulb is a suitable model to elucidate the priming conditions and functional involvement of Fos protein synthesis triggered by physiological sensory stimulation.
使用一种选择性单克隆Fos抗体，我们研究了清醒大鼠嗅球中c-fos原癌基因在丙酸蒸汽刺激下的表达。我们完善了一种方法，该方法能够比较同一嗅球中细胞c-fos免疫反应性模式或肾小球2-脱氧葡萄糖摄取模式。c-fos在局部嗅球中间神经元中表达，主要是颗粒细胞。我们观察到，在由高2-脱氧葡萄糖肾小球摄取灶所界定的嗅球柱中，嗅觉刺激下基础c-fos表达的低水平显著升高。这是首次证明在正常呼吸的清醒大鼠中，传入嗅觉输入可在嗅球中触发c-fos表达。这些数据支持这样一种假设，即嗅球是阐明由生理感觉刺激触发的Fos蛋白合成的启动条件和功能参与的合适模型。
