Martelli G, Daidone M G, Mastore M, Gabrielli G M, Galante E, Pilotti S, Silvestrini R
Division of Oncologia Diagnostica, Istituto Nazionale per la Studio e la Cura dei Tumori, Milan, Italy.
Cancer. 1993 Apr 15;71(8):2522-7. doi: 10.1002/1097-0142(19930415)71:8<2522::aid-cncr2820710815>3.0.co;2-7.
DNA ploidy and cell kinetics determined by flow-cytometric (FCM) analysis have provided relevant information on the natural history of breast cancer. These highly feasible and evaluable assays are particularly appropriate for small samples from fine-needle aspirates (FNA) to complement cytologic information for diagnosis of breast lesions.
DNA content and the percentage of cells in the cell cycle phases were evaluated on FCM DNA histograms from 281 consecutive FNA of benign and malignant breast lesions.
FCM analysis was possible in 93% of FNA. Qualitative concordance in DNA content between FNA and surgical specimens was observed in 82% of the patients. Of the 145 aneuploid FNA, 144 (99%) were histologically diagnosed as carcinomas. The presence of only diploid clones was observed in 115 FNA: rapidly proliferating (S+G2M > 12%) diploid lesions proved to be histologically malignant in 67% of patients, and slowly proliferating diploid lesions were histologically benign in 79% of patients. False-positive results of ploidy and cell kinetics were observed in 1 and 10 patients, respectively. Of the 163 cancers evaluable for cytology and FCM, 158 were positive at cytology. The presence of aneuploid or of diploid rapidly proliferating clones in cytologically false-negative aspirates resulted in the detection of four additional cancers.
Flow cytometric analysis of ploidy and cell kinetics on FNA could be a valuable tool complementary to cytology for breast cancer diagnosis.
通过流式细胞术（FCM）分析确定的DNA倍性和细胞动力学为乳腺癌的自然史提供了相关信息。这些高度可行且可评估的检测方法特别适用于细针穿刺抽吸活检（FNA）获取的小样本，以补充乳腺病变诊断的细胞学信息。
对281例连续的乳腺良恶性病变FNA的FCM DNA直方图评估DNA含量及细胞周期各时相的细胞百分比。
93%的FNA可行FCM分析。82%的患者FNA与手术标本的DNA含量存在定性一致性。在145例非整倍体FNA中，144例（99%）经组织学诊断为癌。115例FNA仅观察到二倍体克隆：快速增殖（S+G2M>12%）的二倍体病变在67%的患者中经组织学诊断为恶性，而缓慢增殖的二倍体病变在79%的患者中经组织学诊断为良性。分别在1例和10例患者中观察到倍性和细胞动力学的假阳性结果。在163例可进行细胞学和FCM评估的癌症中，158例细胞学检查呈阳性。在细胞学假阴性的抽吸物中存在非整倍体或二倍体快速增殖克隆，使得又检测出4例癌症。
FNA的倍性和细胞动力学的流式细胞术分析可能是一种对乳腺癌诊断有价值的、可补充细胞学检查的工具。
