Daidone M G, Martelli G, Pilotti S, Luisi A, Fariselli G, Coopmans de Yoldi G, de Yoldi G C, Silvestrini R
Oncologia Sperimentale C, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy.
Cytometry. 1995 Sep 15;22(3):177-80. doi: 10.1002/cyto.990220304.
A total of 606 consecutive patients with palpable breast lesions underwent physical examination, mammography, and fine-needle aspiration for cytologic and flow-cytometric (FCM) DNA analyses. FCM determinations included DNA ploidy and the fraction of cells in S + G2 + M phases. Aneuploid clones were considered indicative of malignancy; diploid, rapidly proliferating (S+G2+M>12%) clones were considered suggestive of malignancy; and diploid, slowly proliferating clones were considered indicative of benignancy. Sensitivity, specificity, and the predictive accuracy for positive or negative results of FCM information were 82%, 90%, 93.1% and 69.8%, respectively. These values were lower than those observed for the three conventional diagnostic assays owing to the presence of false-positive results (in 23 cases) or unassessable DNA plots (in 90 cases). FCM information alone detected five cases in which the other tests gave inconclusive results and in association to the conventional diagnostic triple test, increased the incidence of positive conclusive cases from 97.9% to 99.2%. However, further refinements are needed before this approach can be used as a routine diagnostic tool.
共有606例可触及乳腺病变的连续患者接受了体格检查、乳腺X线摄影以及用于细胞学和流式细胞术（FCM）DNA分析的细针穿刺。FCM检测包括DNA倍体以及处于S + G2 + M期的细胞比例。非整倍体克隆被认为提示恶性肿瘤；二倍体、快速增殖（S+G2+M>12%）克隆被认为提示恶性肿瘤；二倍体、缓慢增殖克隆被认为提示良性。FCM信息的阳性或阴性结果的敏感性、特异性和预测准确性分别为82%、90%、93.1%和69.8%。由于存在假阳性结果（23例）或无法评估的DNA图谱（90例），这些值低于三种传统诊断检测所观察到的值。仅FCM信息就检测出5例其他检测结果不确定的病例，并且与传统诊断三联检测相结合，使阳性确诊病例的发生率从97.9%提高到99.2%。然而，在这种方法可作为常规诊断工具使用之前，还需要进一步完善。
