Ehlich A, Schaal S, Gu H, Kitamura D, Müller W, Rajewsky K
Institute for Genetics, University of Cologne, Federal Republic of Germany.
Cell. 1993 Mar 12;72(5):695-704. doi: 10.1016/0092-8674(93)90398-a.
The compartment of mouse B cell progenitors can be resolved into five developmentally related fractions by multicolor flow cytometry. Using this system and employing mutant mice in which the membrane exon of the mu chain, the lambda 5 gene, or the JH locus was inactivated by gene targeting, we found that expression of the pre-B cell receptor complex is necessary for the transition from the large CD43+ to the small CD43- pre-B cell stage. We report the occurrence of immunoglobulin heavy and light chain gene rearrangement at the stage of large B cell precursors. We show that neither the pre-B cell receptor complex nor any gene rearrangement in the heavy chain locus is required for the induction of kappa light chain gene rearrangement in early B cell progenitors.
通过多色流式细胞术，小鼠B细胞祖细胞区室可被解析为五个与发育相关的组分。利用该系统并使用通过基因打靶使μ链膜外显子、λ5基因或JH基因座失活的突变小鼠，我们发现前B细胞受体复合物的表达对于从大的CD43+前B细胞阶段向小的CD43-前B细胞阶段的转变是必需的。我们报道了在大B细胞前体阶段免疫球蛋白重链和轻链基因重排的发生。我们表明，早期B细胞祖细胞中κ轻链基因重排的诱导既不需要前B细胞受体复合物，也不需要重链基因座中的任何基因重排。
