Jung J, Rapp J
Department of Biological Sciences, SUNY College of Optometry, NY 10010.
CLAO J. 1993 Jan;19(1):47-9. doi: 10.1097/00140068-199301000-00008.
New, never-worn, individual hydrophilic contact lenses were incubated in an artificial tear solution (containing lysozyme, albumin, lactoferrin, and glycoprotein) for 24 hours at 37 degrees C with constant stirring. These lenses were then cleaned following the manufacturer's instructions with one of six commercial cleaning systems: AOSEPT, CONSEPT, Oxysept, ReNu, Opti-Free and thermal disinfection in conjunction with the Allergan Enzymatic Contact Lens Cleaner. The protein remaining on each lens after cleaning was removed and then quantified by the Bio-Rad Protein Assay. High resolution gel electrophoresis was used to assess the individual protein profile patterns. We found that only one-third to one-half of the protein deposited on a lens is removed by the above cleaning systems. Of the proteins in the artificial tear solution only lysozyme is removed by cleaning, while lactoferrin, albumin, and glycoprotein tend to remain on the lens. Since many of the complications experienced by contact lens wearers are thought to be related to protein deposits on their lenses, our results suggest the need for more effective contact lens cleaning solutions.
将全新、从未佩戴过的单个亲水性隐形眼镜在含有溶菌酶、白蛋白、乳铁蛋白和糖蛋白的人工泪液中于37℃下持续搅拌孵育24小时。然后按照制造商的说明，使用六种商业清洁系统之一对这些镜片进行清洁：AOSEPT、CONSEPT、Oxysept、ReNu、Opti-Free以及结合爱尔康酶促隐形眼镜清洁液进行热消毒。清洁后，去除残留在每个镜片上的蛋白质，然后通过Bio-Rad蛋白质测定法进行定量。使用高分辨率凝胶电泳来评估各个蛋白质谱模式。我们发现，上述清洁系统只能去除沉积在镜片上三分之一到二分之一的蛋白质。在人工泪液中的蛋白质中，清洁过程仅能去除溶菌酶，而乳铁蛋白、白蛋白和糖蛋白往往会残留在镜片上。由于隐形眼镜佩戴者经历的许多并发症被认为与镜片上的蛋白质沉积物有关，我们的结果表明需要更有效的隐形眼镜清洁液。
