Kiel J S
Kiel Laboratories, Inc., Gainsville, Georgia.
Clin Ther. 1993 Jan-Feb;15(1):30-5.
The purpose of this study was to determine whether protein deposits on soft contact lens could be removed without digital or enzymatic cleaning. Group IV soft contact lenses were incubated in an artificial tear solution of lysozyme, albumin, mucin, calcium chloride, and sodium phosphate. The amount of protein on the lenses was determined using a total protein assay with ninhydrin derivation. Mean (+/- SD) protein levels were significantly higher on untreated lenses (598 +/- 184 micrograms) than on lenses treated with hydrogen peroxide and a catalytic disc (360 +/- 51 micrograms). These findings are discussed in light of the finding that one third of contact lens wearers do not digitally clean their lenses after each use and one half do not use an enzymatic cleaner at least once a week. It is concluded that hydrogen peroxide/catalytic disc-based soft contact lens disinfection can remove up to 40% of the protein deposited on soft contact lenses.
本研究的目的是确定是否可以在不进行手动或酶清洁的情况下去除软性隐形眼镜上的蛋白质沉积物。将IV组软性隐形眼镜置于含有溶菌酶、白蛋白、粘蛋白、氯化钙和磷酸钠的人工泪液溶液中进行孵育。使用茚三酮衍生化的总蛋白测定法测定镜片上的蛋白量。未经处理的镜片（598±184微克）上的平均（±标准差）蛋白水平显著高于用过氧化氢和催化盘处理的镜片（360±51微克）。鉴于三分之一的隐形眼镜佩戴者在每次使用后不进行手动清洁镜片，且二分之一的人每周至少不使用一次酶清洁剂这一发现，对这些结果进行了讨论。得出的结论是，基于过氧化氢/催化盘的软性隐形眼镜消毒可去除沉积在软性隐形眼镜上高达40%的蛋白质。
