一种用于直接分析血浆中链脂肪酸的快速气相色谱法。

A rapid GLC method for the direct analysis of plasma medium chain fatty acids.

作者信息

Mingrone G, De Gaetano A, Greco A V, Capristo E, Raguso C, Tataranni P A, Castagneto M

机构信息

Istituto di Clinica Medica, Università Cattolica del Sacro Cuore, Roma, Italy.

出版信息

Clin Chim Acta. 1993 Jan 31;214(1):21-30. doi: 10.1016/0009-8981(93)90299-j.

DOI:10.1016/0009-8981(93)90299-j

PMID:8453775

Abstract

A new, rapid, gas-liquid-chromatographic (GLC) method for the direct determination of plasma medium-chain fatty acids (MCFA) (from C8 to C12) which does not require derivatization procedures is described. Analysis of underivatized MCFA by GLC showed a detection limit ranging from 0.50 to 0.217 microgram depending on chain length: the longer the chain, the higher the detection limit. Compared with the HPLC and GLC methods currently described in the literature, this GLC procedure appears to be easy, adequately sensitive, accurate and well reproducible for those MCFA, like n-octanoic and n-decanoic acids, present in plasma after p.o. or i.v. MCT administration and it requires a short time (about 30 min) for complete processing.

摘要

本文描述了一种新的、快速的气液色谱（GLC）方法，用于直接测定血浆中链脂肪酸（MCFA，碳链长度为C8至C12），该方法无需衍生化步骤。通过GLC对未衍生化的MCFA进行分析，结果显示检测限在0.50至0.217微克之间，具体取决于链长：链越长，检测限越高。与目前文献中描述的HPLC和GLC方法相比，对于口服或静脉注射中链甘油三酯（MCT）后血浆中存在的那些MCFA，如正辛酸和正癸酸，该GLC方法似乎简便、灵敏度足够、准确且重现性良好，并且整个分析过程耗时较短（约30分钟）。