一种改进的气相色谱法，用于快速同时分析血浆中的中链脂肪酸和中链甘油三酯。

An improved GLC method for a rapid, simultaneous analysis of both medium chain fatty acids and medium chain triglycerides in plasma.

作者信息

Mingrone G, Greco A V, Capristo E, Benedetti G, Castagneto M, Gasbarrini G

机构信息

Istituto di Clinica Medica, Universita Cattolica del S. Cuore, Rome, Italy.

出版信息

Clin Chim Acta. 1995 Sep 15;240(2):195-207. doi: 10.1016/0009-8981(95)06142-0.

DOI:10.1016/0009-8981(95)06142-0

PMID:8548929

Abstract

An improved gas-liquid chromatographic (GLC) method for the direct, simultaneous analysis of both medium chain monocarboxylic acids (MCFA) and medium chain triglycerides (MCT) is reported. The calibration curve of MCFA and MCT is linear in the range from 30 ng to 1000 ng. Five nanograms for tricaproin (MCT-6), 10 ng for tricaprylin (MCT-8) and 15 ng for tricaprin (MCT-9) represent the GLC detection limits of MCT, while those of MCFA range from 40 to 15 ng depending on their chain length: the longer the chain length, the higher the detection limit. The recovery of MCFA range from 79 to 99% and that of MCT from 85 to 99%. An example of plasma concentration curves of MCT and MCFA after an intravenous bolus injection of an MCT emulsion (100 mg MCT/kgbw) in a patient with Crohn's disease is shown.

摘要

本文报道了一种改进的气相色谱（GLC）方法，可直接同时分析中链单羧酸（MCFA）和中链甘油三酯（MCT）。MCFA和MCT的校准曲线在30 ng至1000 ng范围内呈线性。三己精（MCT-6）的检测限为5 ng，三辛酸甘油酯（MCT-8）为10 ng，三癸精（MCT-9）为15 ng，这些代表了MCT的GLC检测限，而MCFA的检测限根据其链长在40至15 ng范围内：链长越长，检测限越高。MCFA的回收率在79%至99%之间，MCT的回收率在85%至99%之间。展示了一名克罗恩病患者静脉推注MCT乳剂（100 mg MCT/kgbw）后MCT和MCFA的血浆浓度曲线示例。