Premawansa S, Snewin V A, Khouri E, Mendis K N, David P H
Open University of Sri Lanka, Nawala, Colombo.
Exp Parasitol. 1993 Mar;76(2):192-9. doi: 10.1006/expr.1993.1022.
The merozoite surface protein MSP1, which is one of the most promising candidates for a malaria vaccine directed against erythrocytic stages, has been shown to be polymorphic in different malarial species. Characterization of the Plasmodium vivax MSP1 gene (Pv200) in two strains (Belem and Salvador-1) revealed the existence of several polymorphic regions. One of these regions has been examined here in primary parasite isolates obtained from patients in Sri Lanka. Oligonucleotide primers hybridizing to conserved parts of the gene on either side of a polymorphic region were used to amplify DNA from 22 isolates. Sequence analysis of the amplified portion of the MSP1 gene in five patients showed the existence of three types of polymorphic regions. Two were almost identical either to that of the Belem or to that of the Salvador-1 strain. The third polymorphic type appeared to have resulted from recombination between the two others. This recombination event took place inside a repeated part of the sequence.
裂殖子表面蛋白1（MSP1）是针对红细胞期疟疾疫苗最有前景的候选抗原之一，已证实在不同疟原虫种类中具有多态性。对间日疟原虫MSP1基因（Pv200）在两个菌株（贝伦株和萨尔瓦多-1株）中的特性分析揭示了几个多态性区域的存在。本文对从斯里兰卡患者分离的原始疟原虫中其中一个区域进行了研究。使用与多态性区域两侧基因保守部分杂交的寡核苷酸引物，从22个分离株中扩增DNA。对5名患者MSP1基因扩增部分的序列分析显示存在三种类型的多态性区域。其中两种与贝伦株或萨尔瓦多-1株的几乎相同。第三种多态性类型似乎是由其他两种之间的重组产生的。这种重组事件发生在序列的重复部分内。
