Gutierrez A, Vicini J, Patarroyo M E, Murillo L A, Patarroyo M A
Instituto de Immunologia, Hospital San Juan de Dios, Universidad Nacional de Columbia, Santafé de Bogotá D.C., Columbia.
Exp Parasitol. 2000 Jul;95(3):215-9. doi: 10.1006/expr.2000.4534.
The Plasmodium vivax merozoite surface protein-1 (PvMSP-1) has been considered a candidate for a malaria vaccine against erythrocytic stages. PvMSP-1 is immunogenic during natural infections and exhibits antigenic polymorphism. The extent of genetic polymorphism in a region between the so-called interspecies conserved blocks (ICBs) 2 and 4 of the PvMSP-1 was analyzed in 20 isolates taken from patients from two different areas in Colombia. Variation is unevenly distributed along this gene segment among the isolates. Comparative analysis of these sequences led to the definition of five sequence types (ST1 to 5). ST1 to ST4 exhibit a variation pattern associated with sequences present in the Salvador or Belem sequences. However, ST5 has clusters of sequence that have not been previously described. The changes found along the five variants confirm the important role of recombinational and/or gene conversion events in generating allelic diversity.
间日疟原虫裂殖子表面蛋白-1（PvMSP-1）被认为是一种针对红细胞阶段的疟疾疫苗候选物。PvMSP-1在自然感染期间具有免疫原性，并表现出抗原多态性。对从哥伦比亚两个不同地区的患者中分离出的20株菌株，分析了PvMSP-1所谓种间保守区（ICB）2和4之间区域的遗传多态性程度。变异在这些分离株的该基因片段上分布不均。对这些序列的比较分析确定了五种序列类型（ST1至5）。ST1至ST4表现出与萨尔瓦多或贝伦序列中存在的序列相关的变异模式。然而，ST5具有以前未描述过的序列簇。在这五个变体中发现的变化证实了重组和/或基因转换事件在产生等位基因多样性中的重要作用。
