Olofsson A, Hebert H, Thelestam M
Center for Structural Biochemistry, Karolinska Institutet, NOVUM, Huddinge, Sweden.
FEBS Lett. 1993 Mar 15;319(1-2):125-7. doi: 10.1016/0014-5793(93)80050-5.
The cytolysin Perfringolysin O was applied to lipid layers and the obtained ring-shaped oligomers analyzed by electron microscopy and image processing. The final result shows the periodic repeat of 2.4 nm along the outer rim of the ring. The asymmetric protein unit, corresponding to one monomer, spans the ring from the convex to the concave surface. It shows a clear protein peak close to the outer radius and less density in the middle of the oligomer. The number of monomers in the average ring is 50, and the inner radius of the aggregate is approximately 15 nm.
将溶细胞素产气荚膜梭菌溶素O应用于脂质层，并通过电子显微镜和图像处理分析所获得的环形寡聚体。最终结果显示，沿着环的外边缘有2.4纳米的周期性重复。对应于一个单体的不对称蛋白质单元从凸面到凹面跨越环。它在靠近外半径处显示出一个清晰的蛋白质峰，而在寡聚体中间密度较低。平均环中单体的数量为50个，聚集体的内半径约为15纳米。
