Brecht M, Kellermann J, Plückthun A
Genzentrum der Universität München, Germany.
FEBS Lett. 1993 Mar 15;319(1-2):84-9. doi: 10.1016/0014-5793(93)80042-s.
The nucleotide sequence of the large subunit E of glutamate mutase of Clostridium tetanomorphum was determined. The protein consists of 483 amino acids and is not made in a precursor form, thus excluding the possibility of subunit E being a pyruvoyl enzyme. It shows no homology to any other protein in the database, and while binding coenzyme B12, a conspicuous B12 binding motif, shared amongst other proteins, is not detectable at the sequence level.
已确定破伤风梭状芽孢杆菌谷氨酸变位酶大亚基E的核苷酸序列。该蛋白质由483个氨基酸组成，并非以前体形式合成，因此排除了亚基E是吡哆醛酶的可能性。它与数据库中的任何其他蛋白质均无同源性，并且在结合辅酶B12时，在序列水平上未检测到其他蛋白质中常见的明显的B12结合基序。
