Holloway D E, Marsh E N
Department of Biochemistry, University of Cambridge, UK.
FEBS Lett. 1993 Feb 8;317(1-2):44-8. doi: 10.1016/0014-5793(93)81488-l.
The gene encoding component E, the large subunit, of adenosylcobalamin (coenzyme B12)-dependent glutamate mutase from Clostridium tetanomorphum has been cloned and sequenced. The mutE gene encodes a protein of 485 amino acid residues, with M(r) 53,708. The mutE gene is situated some 1,400 bp downstream of the mutS gene, which encodes the small subunit of glutamate mutase. Between the two is an open reading frame encoding a protein of 462 amino acids, with M(r) 50,171, and of unknown function. All three genes appear to be transcribed as an operon and lie immediately upstream of the gene encoding beta-methylaspartase, the next enzyme in the pathway of glutamate fermentation. Local homology exists between mutE and a region of beta-methylaspartase which contains an active-site serine residue.
破伤风梭状芽孢杆菌中依赖腺苷钴胺素（辅酶B12）的谷氨酸变位酶的大亚基成分E的编码基因已被克隆并测序。mutE基因编码一个含有485个氨基酸残基的蛋白质，分子量为53,708。mutE基因位于mutS基因下游约1400bp处，mutS基因编码谷氨酸变位酶的小亚基。两者之间有一个开放阅读框，编码一个含有462个氨基酸、分子量为50,171的蛋白质，其功能未知。这三个基因似乎作为一个操纵子进行转录，并且紧邻编码β-甲基天冬氨酸酶（谷氨酸发酵途径中的下一个酶）的基因上游。mutE与β-甲基天冬氨酸酶中含有活性位点丝氨酸残基的区域存在局部同源性。
