用分光光度法评估抗淋巴细胞血清的淋巴细胞毒性活性。
The estimation of lymphotoxic activity of ALS by spectrophotometric method.
作者信息
Gościcka T, Rudnicka W, Tarabasz L
出版信息
J Immunol Methods. 1977;15(1):9-15. doi: 10.1016/0022-1759(77)90012-6.
PMID:845443
Abstract
Saijo's method of quantitation of the cytotoxicity of antiserum for tumor cells is adapted to estimation of lymphotoxic activity of antilymphocyte sera. The method is based on spectrophotometric measurement of an amount of trypan blue dye entering the lymphocytes damaged by action of ALS. Several possibilities of determination of the lymphotoxic titers of ALS are suggested.
摘要
斋藤用于定量抗血清对肿瘤细胞细胞毒性的方法被应用于评估抗淋巴细胞血清的淋巴毒性活性。该方法基于分光光度法测量锥虫蓝染料进入因抗淋巴细胞血清作用而受损的淋巴细胞的量。文中提出了几种测定抗淋巴细胞血清淋巴毒性效价的可能性。
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