Bonne C, Raynaud J P
J Invest Dermatol. 1977 Apr;68(4):215-20. doi: 10.1111/1523-1747.ep12493243.
The costovertebral organs [CVO] and seminel vesicles [SV] of the hamster exhibit high saturable androgen uptake. The physicochemcal characteristics of the cytoplasmic androgen receptor present in these tissues have been determined and compared to those obtained in rat prostate[P]. Using the synthetic androgen R 1881 [methyltrienolone] as a radioactive ligand, it has been shown that the affinity of this compound for the cytosol CVO receptor [Kd = 0.7 +/- 0.1 nM] is similar to that for the crytosol SV receptor [Kd = 2.4 +/- 0.9 nm] in hamsters and the cytosol P receptor [Kd = 0.6 +/- 0.1 nM] in rats. The hormonal specificity of binding in these tissues is restricted to androgens. Moreover, testosterone and dihydrotestosterone have the same relative binding affinity in CVO and SV compared to R 1881. Following castration, the total number of androgen sites, as measured by an exchange assay with R 1881, decreases rapidly and parallels with a fall in lipogenic activity. Administration of an androgen rapidly restores binding capacity.
仓鼠的肋椎器官（CVO）和精囊（SV）表现出高饱和性雄激素摄取。已确定这些组织中存在的细胞质雄激素受体的物理化学特性，并与在大鼠前列腺（P）中获得的特性进行了比较。使用合成雄激素R1881（甲基三烯olone）作为放射性配体，已表明该化合物对仓鼠细胞质CVO受体（Kd = 0.7 +/- 0.1 nM）的亲和力与对仓鼠细胞质SV受体（Kd = 2.4 +/- 0.9 nM）以及对大鼠细胞质P受体（Kd = 0.6 +/- 0.1 nM）的亲和力相似。这些组织中结合的激素特异性仅限于雄激素。此外，与R1881相比，睾酮和双氢睾酮在CVO和SV中具有相同的相对结合亲和力。去势后，通过与R1881的交换测定法测量的雄激素位点总数迅速减少，且与脂肪生成活性的下降平行。给予雄激素可迅速恢复结合能力。
