Portnoy J, Pacheco F, Barnes C, Upadrashta B, Crenshaw R, Esch R
Section of Allergy/Immunology, Children's Mercy Hospital, Kansas City, MO 64108.
J Allergy Clin Immunol. 1993 Mar;91(3):773-82. doi: 10.1016/0091-6749(93)90197-n.
In an attempt to identify representative strains, 12 strains of Alternaria alternata were contributed by four different research groups. Each was grown on two types of solid media and characterized with descriptions of pigmentation and morphology. Enzyme profiles were determined on both the cellular antigen and the culture filtrate material with use of a commercial kit. Concentrations of a previously described Alt a 1 and a 70 kd allergen, GP70, were measured by a two-antibody "sandwich" ELISA. Allergenic proteins were visualized by IgE immunoblots. With use of these criteria, four separate strains were identified that might serve as representative of Alternaria for further research.
为了鉴定代表性菌株，四个不同的研究小组提供了12株链格孢菌。每株菌都在两种固体培养基上培养，并根据色素沉着和形态描述进行了特征鉴定。使用商业试剂盒测定了细胞抗原和培养滤液材料的酶谱。通过双抗体“夹心”ELISA法测定了先前描述的Alt a 1和一种70kd变应原GP70的浓度。通过IgE免疫印迹法观察变应原蛋白。根据这些标准，鉴定出了四个不同的菌株，它们可能作为链格孢菌的代表用于进一步研究。
