Toh H, Miyata T, Torisu M
First Department of Surgery, Kyushu University School of Medicine, Fukuoka, Japan.
J Leukoc Biol. 1993 Mar;53(3):256-63. doi: 10.1002/jlb.53.3.256.
The relationship between activated neutrophils and end-organ injury in endotoxemia was studied. The function of peripheral blood neutrophils (PMNs) in rabbits with the generalized Shwartzman reaction (GSR) was compared to that of PMNs rabbits receiving a single injection of endotoxin. The following results were obtained: (1) PMNs from rabbits with the GSR demonstrated enhanced adherence to endothelial cells and increased mitochondrial ATP production; (2) the GSR did not enhance chemotaxis and oxygen radical production of PMNs; (3) a single injection of endotoxin did not cause necrosis of visceral organs; (4) in vitro detachment of endothelial cells by PMNs was increased in rabbits with the GSR; (5) in vivo administration of monoclonal antibody (mAb) against CD11b/CD18 (Mac-1) suppressed the increase in PMN adherence; and (6) hemorrhagic necrosis did not occur when mAb to Mac-1 was injected. Thus, enhanced adherence of PMNs to endothelial cells appears to play a key role in endotoxin-induced end-organ injuries in this animal model.
研究了内毒素血症中活化中性粒细胞与靶器官损伤之间的关系。将发生全身性施瓦茨曼反应（GSR）的家兔外周血中性粒细胞（PMN）的功能与单次注射内毒素的家兔的PMN功能进行了比较。得到以下结果：（1）发生GSR的家兔的PMN对内皮细胞的黏附增强，线粒体ATP生成增加；（2）GSR未增强PMN的趋化性和氧自由基生成；（3）单次注射内毒素未导致内脏器官坏死；（4）发生GSR的家兔中，PMN介导的内皮细胞体外脱离增加；（5）体内给予抗CD11b/CD18（Mac-1）单克隆抗体（mAb）可抑制PMN黏附的增加；（6）注射抗Mac-1 mAb时未发生出血性坏死。因此，在该动物模型中，PMN对内皮细胞黏附的增强似乎在内毒素诱导的靶器官损伤中起关键作用。
