Messenguy F, Dubois E
Institut de Recherches du CERIA, Brussels, Belgium.
Mol Cell Biol. 1993 Apr;13(4):2586-92. doi: 10.1128/mcb.13.4.2586-2592.1993.
ARGRI, ARGRII, and ARGRIII regulatory proteins control the expression of arginine anabolic and catabolic genes in Saccharomyces cerevisiae. We have shown that MCM1 is part of the ARGR regulatory complex, by in vitro binding experiments, at the ARGR5,6 promoter. The participation of MCM1 in the regulation of arginine metabolism is confirmed by the behavior of an mcm1-gcn4 mutant, which is affected in the repression of arginine anabolic genes. In this mcm1 mutant, synthesis of the catabolic enzymes is rather constitutive, but this derepression requires the integrity of the ARGR system and of the target sequences of these proteins in the CAR1 promoter. Our in vitro binding experiments confirm the presence of MCM1 in the protein complex interacting with the promoters of the catabolic CAR1 and CAR2 genes. This is the first in vivo transcription role ascribed to MCM1 other than its role in the transcription of cell-type-specific genes.
ARGRI、ARGRII和ARGRIII调节蛋白控制酿酒酵母中精氨酸合成代谢和分解代谢基因的表达。我们通过体外结合实验表明，MCM1是ARGR调节复合物的一部分，位于ARGR5,6启动子处。mcm1 - gcn4突变体的行为证实了MCM1参与精氨酸代谢的调节，该突变体在精氨酸合成代谢基因的抑制方面受到影响。在这个mcm1突变体中，分解代谢酶的合成相当组成型，但这种去抑制需要ARGR系统以及这些蛋白在CAR1启动子中的靶序列的完整性。我们的体外结合实验证实了MCM1存在于与分解代谢基因CAR1和CAR2启动子相互作用的蛋白质复合物中。这是除了其在细胞类型特异性基因转录中的作用之外，首次赋予MCM1的体内转录作用。
