Dubois E, Messenguy F
Mol Gen Genet. 1985;198(2):283-9. doi: 10.1007/BF00383008.
ARGRII is one of the three regulatory genes controlling arginine metabolism in yeast. From a pool of hybrid plasmids carrying Sau3A fragments representing the entire yeast genome, a DNA fragment containing the regulatory gene ARGRII was cloned by complementation of an argRII- mutation, which prevents growth on ornithine as sole nitrogen source. Cells containing the cloned DNA regained the ability to repress the synthesis of anabolic enzymes and to induce the synthesis of the catabolic ones, when arginine is present. The 6.2 kb cloned DNA fragment encodes five transcripts (2.8 kb, 1.3 kb, 0.75 kb, 0.45 kb, 0.45 kb), which were located by S1 endonuclease mapping. By marker rescue the argRII- mutations were mapped in the DNA region coding for the 2.8 kb transcript, showing its importance in the control mechanism. Subcloning experiments confirm this result. However, at present the role of the 0.75 kb and 1.3 kb transcripts in the ARGR+ phenotype is unclear.
ARGRII是控制酵母中精氨酸代谢的三个调控基因之一。从一组携带代表整个酵母基因组的Sau3A片段的杂交质粒中，通过对argRII-突变（该突变阻止细胞以鸟氨酸作为唯一氮源生长）进行互补，克隆了一个包含调控基因ARGRII的DNA片段。当存在精氨酸时，含有克隆DNA的细胞恢复了抑制合成代谢酶的合成并诱导分解代谢酶合成的能力。这个6.2 kb的克隆DNA片段编码五个转录本（2.8 kb、1.3 kb、0.75 kb、0.45 kb、0.45 kb），通过S1核酸酶作图确定了它们的位置。通过标记拯救，将argRII-突变定位在编码2.8 kb转录本的DNA区域，表明其在控制机制中的重要性。亚克隆实验证实了这一结果。然而，目前尚不清楚0.75 kb和1.3 kb转录本在ARGR+表型中的作用。
