Werner M, Feller A, Messenguy F, Piérard A
Cell. 1987 Jun 19;49(6):805-13. doi: 10.1016/0092-8674(87)90618-0.
The expression of gene CPA1, encoding the glutaminase subunit of the arginine pathway carbamoyl-phosphate synthetase, is repressed by arginine at a posttranscriptional level. The 5' region of CPA1 mRNA contains a 25 codon upstream open reading frame. The importance of this feature for the repression of CPA1 expression has been analyzed by oligonucleotide-directed mutagenesis and by sequencing of constitutive cis-dominant mutations obtained in vivo. The results show that the leader peptide, the product of the upstream open reading frame, plays an essential, negative role in the specific repression of CPA1 by arginine. A model of translational regulation of CPA1 is proposed that takes into account the cis-dominance of the mutations affecting the leader peptide.
编码精氨酸途径氨基甲酰磷酸合成酶谷氨酰胺酶亚基的基因CPA1的表达在转录后水平受到精氨酸的抑制。CPA1 mRNA的5'区域包含一个25密码子的上游开放阅读框。通过寡核苷酸定向诱变以及对体内获得的组成型顺式显性突变进行测序，分析了该特征对于CPA1表达抑制的重要性。结果表明，上游开放阅读框的产物前导肽在精氨酸对CPA1的特异性抑制中起重要的负向作用。提出了一个CPA1翻译调控模型，该模型考虑了影响前导肽的突变的顺式显性。
