Jensen H E, Frandsen P L, Schønheyder H
Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Zentralbl Veterinarmed B. 1993 Feb;40(1):55-65. doi: 10.1111/j.1439-0450.1993.tb00109.x.
ELISA and immunoblotting were applied for the characterization of somatic antigens from Absidia corymbifera. Immunoblotting revealed major antigenic bands at 11 to 81 kDa. The ELISA showed some crossreactivity towards somatic antigens from other fungi. However, the crossreactivity was especially observed with somatic antigens from other fungi of the zygomycetes. The ELISA and immunoblotting assays were applied to urine samples from two groups of 3 calves each systemically infected with A. corymbifera and Aspergillus fumigatus, respectively. The immunoreactivity of the urine samples was similar by the two assays. Somatic antigens were demonstrated in the urine of all three calves infected with A.corymbifera, whereas only one of the calves with systemic aspergillosis was antigen positive. The level of antigen in the positive urine samples varied from 50 to 210 ng/ml.
采用酶联免疫吸附测定（ELISA）和免疫印迹法对伞枝犁头霉的体细胞抗原进行特性分析。免疫印迹法显示在11至81 kDa处有主要抗原条带。ELISA显示对其他真菌的体细胞抗原有一定交叉反应性。然而，这种交叉反应性在接合菌纲的其他真菌的体细胞抗原中尤为明显。ELISA和免疫印迹法应用于分别被伞枝犁头霉和烟曲霉全身感染的两组三头犊牛的尿液样本。两种检测方法显示尿液样本的免疫反应性相似。在所有三头被伞枝犁头霉感染的犊牛尿液中均检测到体细胞抗原，而在全身感染曲霉菌病的犊牛中只有一头抗原呈阳性。阳性尿液样本中的抗原水平在50至210 ng/ml之间。
