Dipartimento di Salute Animale, Parma University, 43126 Parma, Italy.
Reprod Biol Endocrinol. 2009 Nov 30;7:138. doi: 10.1186/1477-7827-7-138.
Lichtheimia corymbifera (previously Absidia corymbifera) is a filamentous zygomycetes belonging to the order Mucorales and to the family Lichtheimiaceae. Members of genus Lichtheimia spp. are cosmopolitan and ubiquitous in nature. Lichtheimia corymbifera is a recognized agent of diseases in man and animals. In cattle it causes abortion and mastitis. Three cases of bovine abortion occurred in a herd located in the Po Valley. Serological examinations were performed on fetal and mother's blood. One of the aborted fetus was referred to our laboratory. The paper describes the isolation and characterization of Lichtheimia corymbifera from a bovine aborted fetus.
Serological examinations were performed on fetal and mother's blood. Lesions on fetal tissues and placenta leaded the diagnostic suspect towards a mycotic aetiology. Tissues were then put in culture, and at the same time an histological examination was performed, together with bacteriological and virological tests. The isolate from placenta and fetal tissues was identified and characterized by PCR and RFLP, using the ITS region as a target sequence and AclI restriction site within the amplicon to distinguish Lichtheimia corymbifera among the other fungi.
Serological, bacteriological and virological tests gave aspecific results. Histological examination evidenced numerous PAS positive hyphae within the necrotic cotiledons and numerous fungal nonseptate hyphae to the GMS stain. Colonies with typical morphological features of fungi grew up on Sabouraud agar from fetal skin and placenta. On the developed colonies the microscopic examination has shown a large number of nonseptate hyphae and sporangia consistent with Mucorales. PCR and RFLP allowed the identification of the isolate as Lichtheimia corymbifera.
The present report describes the isolation and the molecular characterisation of a fungal isolate from bovine aborted fetus and placenta. The diagnostic protocol allowed to identify and characterise the strain. This is the first isolation in Italy of Lichtheimia corymbifera in a bovine aborted fetus.
Lichtheimia corymbifera（以前称为 Absidia corymbifera）是一种丝状接合菌，属于毛霉目和光柄菇科。属 Lichtheimia spp. 的成员在全球范围内普遍存在。Lichtheimia corymbifera 是人类和动物疾病的公认病原体。在牛中，它会导致流产和乳腺炎。在位于波河谷的一个牛群中发生了三例牛流产。对胎儿和母亲的血液进行了血清学检查。其中一只流产的胎儿被送到我们的实验室。本文描述了从牛流产胎儿中分离和鉴定 Lichtheimia corymbifera 的方法。
对胎儿和母亲的血液进行了血清学检查。胎儿组织和胎盘上的病变导致诊断怀疑为真菌病因。然后将组织放入培养物中，同时进行组织学检查，以及细菌学和病毒学检查。从胎盘和胎儿组织中分离出的菌株通过 PCR 和 RFLP 进行鉴定和特征分析，使用 ITS 区域作为靶序列，并使用扩增子中的 AclI 限制性位点来区分接合菌属中的其他真菌 Lichtheimia corymbifera。
血清学、细菌学和病毒学检查结果均无特异性。组织学检查显示坏死的子叶中有大量 PAS 阳性菌丝，GMS 染色显示有大量无隔菌丝。来自胎儿皮肤和胎盘的萨布罗琼脂上长出具有典型真菌形态特征的菌落。在发育良好的菌落上，显微镜检查显示大量无隔菌丝和与毛霉目一致的孢子囊。PCR 和 RFLP 允许鉴定分离株为 Lichtheimia corymbifera。
本报告描述了从牛流产胎儿和胎盘分离真菌的方法以及分子特征。诊断方案允许鉴定和表征菌株。这是意大利首次在牛流产胎儿中分离出 Lichtheimia corymbifera。
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