通过对组织中真菌抗原进行交叉免疫电泳诊断牛全身性曲霉病和毛霉病

Crossed immunoelectrophoresis of fungal antigens in tissues as a means of diagnosing systemic aspergillosis and zygomycosis in cattle.

作者信息

Jensen H E

机构信息

Department of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.

出版信息

Vet Res Commun. 1993;17(4):267-75. doi: 10.1007/BF01839217.

DOI:10.1007/BF01839217

PMID:8146952

Abstract

A novel method for diagnosing bovine aspergillosis and zygomycosis is described. Rabbit hyperimmune antisera raised against somatic antigens of Aspergillus fumigatus and Absidia corymbifera were used in crossed immunoelectrophoresis with supernatants from disintegrated tissues from acute necrohaemorrhagic mycotic lesions from cattle. The method specifically identified 4 of 5 lesions with aspergillosis and 2 of 5 lesions with zygomycosis. One lesion dually infected with aspergillosis and zygomycosis was negative. The method worked with unabsorbed sera, was specific, and required only standard electrophoretic equipment. It can therefore supplement chemical detection of fungi in tissues in the diagnosis of bovine aspergillosis and zygomycosis.

摘要

本文描述了一种诊断牛曲霉菌病和接合菌病的新方法。用针对烟曲霉和伞枝犁头霉体细胞抗原产生的兔超免疫抗血清，与来自牛急性坏死性出血性霉菌性病变组织匀浆的上清液进行交叉免疫电泳。该方法特异性地鉴定出5例曲霉菌病病变中的4例和5例接合菌病病变中的2例。1例同时感染曲霉菌病和接合菌病的病变呈阴性。该方法使用未吸收的血清，具有特异性，仅需标准电泳设备。因此，在牛曲霉菌病和接合菌病的诊断中，它可以补充组织中真菌的化学检测。