Taylor R G, Papa I, Astier C, Ventre F, Benyamin Y, Ouali A
INRA, Muscle Biochemistry Unit SRV, St. Genes Champanelle, France.
J Muscle Res Cell Motil. 1997 Jun;18(3):285-94. doi: 10.1023/a:1018665924412.
Striated muscle cytoskeleton was studied by ultrastructure and electrophoresis. Treatment of sea bass white muscle myofibrils and glycerinated fibres with calpain caused disruption of costameres, intermediate filaments, and Z-line, without altering sarcomeres. V8 protease also caused loss of costameres and Z-line, and disrupted sarcomeres without affecting the intermediate filaments. Recombinant lipase caused loss of Z-lines and also sarcolemma detachment, without changing sarcomeres or intermediate filaments. DNase-1 removed thin filaments and partially removed Z-lines while leaving intact the sarcolemma attachments and intermediate filaments. Calpain, V8 protease, lipase and DNase-1 treatments induced extensive loss of alpha-actinin from the Z-line, which could be related to titin cleavage (calpain, V8), phosphoinositide hydrolysis (lipase), and actin depolymerisation (DNase-1). These results show that the cytoskeletal components are independent of intact thin filaments.
通过超微结构和电泳研究了横纹肌细胞骨架。用钙蛋白酶处理海鲈白肌肌原纤维和甘油化纤维会导致肌小节、中间丝和Z线的破坏，但不会改变肌节。V8蛋白酶也会导致肌小节和Z线的丢失，并破坏肌节而不影响中间丝。重组脂肪酶会导致Z线的丢失以及肌膜脱离，而不会改变肌节或中间丝。脱氧核糖核酸酶-1去除细肌丝并部分去除Z线，同时保持肌膜附着和中间丝完整。钙蛋白酶、V-8蛋白酶、脂肪酶和脱氧核糖核酸酶-1处理导致Z线中α-辅肌动蛋白大量丢失，这可能与肌联蛋白裂解（钙蛋白酶、V8）、磷酸肌醇水解（脂肪酶）和肌动蛋白解聚（脱氧核糖核酸酶-1）有关。这些结果表明细胞骨架成分独立于完整的细肌丝。
