Akanji A O, Famuyiwa O O
Department of Chemical Pathology, College of Medicine, University College Hospital, Ibadan, Nigeria.
Br J Nutr. 1993 Jan;69(1):269-76. doi: 10.1079/bjn19930028.
Intraperitoneal glucose tolerance tests were performed at 4-week intervals in groups of weanling rats before and after feeding with maize- or cassava-based diets with and without adequate protein and sublethal cyanide supplementation. Weaning weights were doubled (increase of about 50 g) after 4 weeks on control (maize-based with adequate protein) and protein-replete diets. Weight gain on the protein-deficient diets was much less (22 g or 50%), a pattern maintained by the rats on these diets until the age of 12 weeks. Plasma thiocyanate levels were identical at weaning and after 8 weeks of the control diet but increased by 200-300% after 4 weeks intake of the cassava or cyanide-supplemented feeds. Levels returned to normal in all groups after a further 4 weeks feeding with the control diet. Glucose tolerance (as assessed by the area under the 2 h glucose v. time curve) was impaired to a varying extent in the rats after 4 weeks on the various diets: protein-replete cassava and protein-deficient maize diets by 50% protein-deficient cassava diet by 300%, and cyanide-supplemented protein-deficient maize diet by 150%. The derangement in the rats on the protein-replete cassava diet was unaffected by a further 4 weeks intake of the control diet, unlike in the other groups where there was significant improvement in the glucose tolerance indices at the same time. It is concluded that in growing rats: (1) cassava intake and protein malnutrition may have independent and additive effects on the genesis of glucose intolerance, (2) cyanide supplementation of a cassava-free protein-replete diet has no effect on glucose tolerance.
在断奶大鼠组中，每隔4周进行一次腹膜内葡萄糖耐量试验，试验在喂食以玉米或木薯为基础的饮食前后进行，这些饮食添加或不添加充足蛋白质以及亚致死量氰化物。在对照（含充足蛋白质的玉米基）和蛋白质充足的饮食喂养4周后，断奶体重翻倍（增加约50克）。蛋白质缺乏饮食的体重增加要少得多（22克或50%），这些饮食组的大鼠一直保持这种模式，直到12周龄。断奶时和对照饮食8周后的血浆硫氰酸盐水平相同，但在摄入木薯或添加氰化物的饲料4周后增加了200 - 300%。再用对照饮食喂养4周后，所有组的水平恢复正常。在各种饮食喂养4周后，大鼠的葡萄糖耐量（通过2小时葡萄糖与时间曲线下面积评估）在不同程度上受损：蛋白质充足的木薯饮食和蛋白质缺乏的玉米饮食受损50%，蛋白质缺乏的木薯饮食受损300%，添加氰化物的蛋白质缺乏玉米饮食受损150%。与其他组在同一时间葡萄糖耐量指数有显著改善不同，蛋白质充足的木薯饮食组大鼠的紊乱情况在再摄入4周对照饮食后没有受到影响。得出的结论是，在生长中的大鼠中：（1）木薯摄入和蛋白质营养不良可能对葡萄糖不耐受的发生有独立且累加的影响，（2）在不含木薯但蛋白质充足的饮食中添加氰化物对葡萄糖耐量没有影响。
